| Generation of the Compression-induced Dedifferentiated Adipocytes (CiDAs) Using Hypertonic Medium
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Author:
Date:
2021-02-20
[Abstract] Current methods to obtain mesenchymal stem cells (MSCs) involve sampling, culturing, and expanding of primary MSCs from adipose, bone marrow, and umbilical cord tissues. However, the drawbacks are the limited numbers of total cells in MSC pools, and their decaying stemness during in vitro expansion. As an alternative resource, recent ceiling culture methods allow the generation of dedifferentiated fat cells (DFATs) from mature adipocytes. Nevertheless, this process of spontaneous dedifferentiation of mature adipocytes is laborious and time-consuming. This paper describes a modified protocol for in vitro dedifferentiation of adipocytes by employing an additional physical stimulation, which takes advantage of augmenting the stemness-related Wnt/β-catenin signaling. Specifically, this ...
[摘要] [摘要]目前的方法，以获得间充质干细胞（MSC）包括采样，培养，和扩大主要由脂肪，骨髓，和脐带组织的MSCs。然而，缺点是在总细胞在MSC池，和它们的衰减干性的数量有限在维生素- [R Ò扩张。作为替代资源，最近的天花板培养方法允许从成熟的脂肪细胞中生成去分化的脂肪细胞（DFAT）。然而，这种成熟脂肪细胞自发去分化的过程既费力又费时。本文描述了一种用于经修改协议在体外通过采用附加的物理刺激，其中脂肪细胞去分化TA KES扩充所述干性相关的优点的Wnt /β-catenin信号。具体来说，该协议利用含聚乙二醇（PEG）的高渗介质引入细胞外物理刺激以获得更高的效率，并引入更简单的脂肪细胞去分化程序。
[背景]脂肪组织由于其丰度大且侵袭性相对较低，因此是间充质干细胞（MSC）最具吸引力的来源之一（Shen等，2011 ；González-Cruz等，2012； Konno等人，2013）。脂肪来源的MSC，即从皮下脂肪组织的基质血管级分中分离，已被证实同时显示多谱系潜能的体外和体内（Anghileri等人，2008;冈萨雷斯。等人，2009;冈萨雷斯-雷伊等等人，2010； Jumabay等人，2010； Mao等人，2017和2019 ；Darnell等人，2018 ...
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| A Streamlined Method for the Preparation of Growth Factor-enriched Thermosensitive Hydrogels from Soft Tissue
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Author:
Date:
2017-02-05
[Abstract] Hydrogels are an ideal medium for the expansion of cells in three dimensions. The ability to induce cell expansion and differentiation in a controlled manner is a key goal in tissue engineering. Here we describe a detailed method for producing hydrogels from soft tissues with an emphasis on adipose tissue. In this method, soluble, extractable proteins are recovered from the tissue and stored while the remaining insoluble tissue is processed and solubilised. Once the tissue has been sufficiently solubilised, the extracted proteins are added. The resulting product is a thermosensitive hydrogel with proteins representative of the native tissue. This method addresses common issues encountered when working with some biomaterials, such as high lipid content, DNA contamination, and finding an ...
[摘要] 水凝胶是三维细胞扩增的理想媒介。以受控方式诱导细胞扩增和分化的能力是组织工程中的关键目标。在这里，我们描述了从软组织生产水凝胶的详细方法，重点是脂肪组织。在该方法中，可溶性可提取蛋白从组织中回收并储存，而剩余的不溶组织被加工和溶解。一旦组织被充分溶解，就加入提取的蛋白质。所得产物是具有代表天然组织的蛋白质的热敏水凝胶。这种方法解决了在使用某些生物材料时遇到的常见问题，例如高脂质含量，DNA污染和找到适当的灭菌方法。尽管本文的重点是脂肪组织，但使用这种方法，我们已经从其他软组织（包括肌肉，肝脏和心脏组织）中产生了水凝胶。
背景 组织工程的主要目标是通过向身体提供具有与目标部位相似性质的支架来产生新的组织。这允许最佳的重塑并且使得能够形成新生内源性组织。在脂肪组织工程领域，来自脂肪组织的生物材料是特别有意义的，因为脂肪组织广泛可用，并且在理论上为脂肪形成诱导提供了最佳可能的环境（Flynn等人，2007; Flynn，2010; Uriel等人，2008; Choi等人，2009; Young等人，2011）。已经确定脂肪细胞分泌脂肪形成因子（Li et al。，1998; Shillabeer等人，1989; Shillabeer等人， ...
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| Isolation and Culture of Human Adipose-derived Stem Cells from Subcutaneous and Visceral White Adipose Tissue Compartments
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Author:
Date:
2016-11-20
[Abstract] Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. In this detailed protocol we describe a protocol to isolate and characterize ASCs from human WAT. Molecular characterization of isolated ASCs was performed through surface marker expression profiling using flow cytometry. Adipogenic capacity of the isolated ASCs was confirmed through inducing adipogenic differentiation and Oil Red O ...
[摘要] Human Adipose-derived Stem/Stromal Cells (ASCs) have been widely used in stem cell and obesity research, as well as clinical applications including cell-based therapies, tissue engineering and reconstruction. Compared with mesenchymal stem cells (MSCs) derived from other tissues such as umbilical cord and bone marrow, isolation of ASCs from human white adipose tissue (WAT) has great advantages due to its rich tissue source and simple surgical procedure. In this detailed protocol we describe a protocol to isolate and characterize ASCs from human WAT. Molecular characterization of isolated ASCs ...
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