Adenosine 5′-diphosphate sodium salt
Company: Sigma-Aldrich
Catalog#: A2754
Product evaluation:
Spectrophotometric Determination of Glutamine Synthetase Activity in Cultured Cells
Author:
Date:
2016-10-05
[Abstract]  Glutamine synthetase (GS), which catalyzes the conversion of glutamate and ammonia to glutamine, is widely distributed in animal tissues and cell culture lines. The importance of this enzyme is suggested by the fact that glutamine, the product of GS-catalyzed de novo synthesis reaction, is the most abundant free amino acid in blood (Smith and Wilmore, 1990). Glutamine is involved in many biological processes including serving as the nitrogen donor for biosynthesis, as an exchanger for the ...

Determination of the H+-ATP Synthase and Hydrolytic Activities
Author:
Date:
2016-08-20
[Abstract]  The H+-ATP synthase of the inner mitochondrial membrane utilizes the proton gradient generated by the respiratory chain to synthesize ATP. Under depolarizing conditions, it can function in reverse by hydrolyzing ATP to generate a proton gradient. The protocols presented here allow the facile determination of both the synthetic and hydrolytic activities of the H+-ATP synthase in isolated mitochondria and in permeabilized mammalian cells. Since the protocol requires the ...

Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
Author:
Date:
2014-08-05
[Abstract]  It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed protocols for the isolation of tomato fruit chromoplasts and the determination of ATP levels (end-point measurements) and ATP synthesis rates (kinetic ...

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