|
Author:
Date:
2012-09-20
[Abstract] The matrigel plug angiogenesis assay is a simple in vivo technique to detect the newly formed blood vessels in the transplanted gel plugs in nude mice. The matrigel matrix is derived from the engelbroth-holm-swarm (EHS) mouse sarcoma, and its composition is comparable to the basement membrane proteins. The matrigel can induce differentiation of a variety of cell types such as hepatocytes, mammary epithelial cells, and endothelial cells. In our case, tumor cells are mixed with the matrigel gel and are injected into the mice. The later immunohistochemistry (IHC) staining with the endothelial marker indicates the presence of the newly formed capillaries in the sectioned gel plugs.
[摘要] 基质胶塞血管生成测定是在裸鼠中检测移植的凝胶栓中新形成的血管的简单的体内技术。 基质胶基质来源于engelbroth-holm-swarm（EHS）小鼠肉瘤，其组成与基底膜蛋白相当。 基质胶可诱导多种细胞类型的分化，例如肝细胞，乳腺上皮细胞和内皮细胞。 在我们的情况下，将肿瘤细胞与基质胶凝胶混合并注射到小鼠中。 用内皮标记物的后期免疫组织化学（IHC）染色表明在切片的凝胶塞中存在新形成的毛细血管。
|