Flavin adenine dinucleotide disodium salt hydrate
Company: Sigma-Aldrich
Catalog#: F6625
Product evaluation:
ER Microsome Preparation and Subsequent IAA Quantification in Maize Coleoptile and Primary Root Tissue
Author:
Date:
2016-05-05
[Abstract]  Auxin is a major growth hormone in plants and the first plant hormone to be discovered and studied (Darwin and Darwin, 1880). The auxin molecule in plants was first identified as indole-3-acetic acid (IAA) by Kögl et al. (1934). Active research over nearly a decade has shed light on many of the molecular mechanisms of its action but the complexity and redundancy of the auxin biosynthetic network raises questions about control of this system. We have shown that some enzymes involved in ...

Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
Author:
Date:
2014-08-05
[Abstract]  It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed protocols for the isolation of tomato fruit chromoplasts and the determination of ATP levels (end-point measurements) and ATP synthesis rates (kinetic ...

Quantification of Flavin Production by Bacteria
Author:
Date:
2014-08-05
[Abstract]  This protocol provides a simple and fast method of quantification for intracellular flavin content, and for flavin secretion by bacteria. Intracellular flavins are extracted from bacterial pellets, and secreted flavins are examined in the cell growth medium. Flavins are separated and measured using HPLC with fluorescence detection, and quantified based on a comparison to standards.

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