β-Nicotinamide adenine dinucleotide hydrate
Company: Sigma-Aldrich
Catalog#: N1636
Product evaluation:
Determination of Recombinant Mannitol-1-phosphate Dehydrogenase Activity from Ectocarpus sp.
Author:
Date:
2016-11-05
[Abstract]  Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of these is the mannitol cycle, which plays a central role in their physiology, as mannitol acts as carbon storage, osmoprotectant, and antioxidant. This ...

Determination of Mannitol-2-dehydrogenase Activity
Author:
Date:
2015-11-05
[Abstract]  Mannitol is a polyol that occurs in a wide range of living organisms, where it fulfills different physiological roles. Several pathways have been described for the metabolism of mannitol by bacteria, including the phosphoenolpyruvate-dependent phosphotransferase system (PST) and a M2DH-based catabolic pathway. The latter involves two enzymes, a mannitol-2-dehydrogenase (EC 1.1.1.67) and a fructokinase (EC 2.7.1.4), and has been identified in different bacteria, e.g., the marine ...

Isolation of Tomato Fruit Chromoplasts and Determination of ATP Levels
Author:
Date:
2014-08-05
[Abstract]  It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed protocols for the isolation of tomato fruit chromoplasts and the determination of ATP levels (end-point measurements) and ATP synthesis rates (kinetic ...

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