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Boric acid

硼酸

Company: Sigma-Aldrich
Catalog#: B0252
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Hippocampal Neuron Dissociation Transfection and Culture in Microfluidics Chambers
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2012-07-20
[Abstract]  Microfluidics chamber is an ideal tool to study local events that occurring in neuronal projections by perfectly compartmentalizing the cell soma from certain branches. It is very well suited for live cell imaging or immunohistochemistry staining. This protocol has been carefully modified in detail to fit the requirement of primary rat hippocampal neuronal cultures. It can also be applied to a more general neuronal culture purpose in microfluidics. [摘要]  微流体室是一个理想的工具,研究发生在神经元投射的本地事件,通过完美地划分细胞体细胞从某些分支。 它非常适合活细胞成像或免疫组织化学染色。 这个协议已经仔细修改,以适应初级大鼠海马神经元文化的要求。 它也可以应用于更一般的神经元文化目的在微流体。

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