β-Nicotinamide adenine dinucleotide phosphate hydrate
Company: Sigma-Aldrich
Catalog#: N5755
Product evaluation:
Determination of Recombinant Mannitol-1-phosphate Dehydrogenase Activity from Ectocarpus sp.
Author:
Date:
2016-11-05
[Abstract]  Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of these is the mannitol cycle, which plays a central role in their physiology, as mannitol acts as carbon storage, osmoprotectant, and antioxidant. This ...

Measurement of Glucose-6-phosphate Dehydrogenase Activity in Bacterial Cell-free Extracts
Author:
Date:
2016-10-05
[Abstract]  Glucose-6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.49) is the first enzyme of the oxidative pentose phosphate cycle and catalyses the conversion of glucose-6-phosphate (G6P) to 6-phosphoglucono-δ-lactone and transfers one electron to NADP+ producing one NADPH. Conversion of G6P to 6-phosphoglucono-δ-lactone is proportional to the production of NADPH. The increase in NADPH concentration results in an increase in absorbance at 340 nm. To assay G6PDH activity, therefore, production of ...

Determination of Mannitol-2-dehydrogenase Activity
Author:
Date:
2015-11-05
[Abstract]  Mannitol is a polyol that occurs in a wide range of living organisms, where it fulfills different physiological roles. Several pathways have been described for the metabolism of mannitol by bacteria, including the phosphoenolpyruvate-dependent phosphotransferase system (PST) and a M2DH-based catabolic pathway. The latter involves two enzymes, a mannitol-2-dehydrogenase (EC 1.1.1.67) and a fructokinase (EC 2.7.1.4), and has been identified in different bacteria, e.g., the marine ...

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