Bovine Serum Albumin
Company: Sigma-Aldrich
Catalog#: A7906
Product evaluation:
Protocol for Establishing a Multiplex Image-based Autophagy RNAi Screen in Cell Cultures
Author:
Date:
2017-09-05
[Abstract]  Autophagy is a recycling pathway, in which intracellular cargoes including protein aggregates and bacteria are engulfed by autophagosomes and subsequently degraded after fusion with lysosomes. Dysregulation of this process is involved in several human diseases such as cancer or neurodegeneration. Hence, advancing our understanding of how autophagy is regulated provides an opportunity to explore druggable targets and subsequently develop treatment strategies for these diseases. To identify novel ...

Isolation and Analysis of Stromal Cell Populations from Mouse Lymph Nodes
Author:
Date:
2017-08-20
[Abstract]  Our protocol describes a simple procedure for isolating stromal cells from lymph nodes (LN). LN are disrupted then enzymatically digested with collagenase and dispase to produce a single cell suspension that can be stained with fluorescently labelled antibodies and analysed by flow cytometry. This protocol will enable identification of fibroblastic reticular cells (FRC), lymphatic endothelial cells (LEC), blood endothelial cells (BEC) as PNAd+ BEC that form LN high endothelial ...

Improved Oviduct Transfer Surgery for Genetically Modified Rat Production
Author:
Date:
2017-08-20
[Abstract]  Rat embryo transfer surgeries are becoming more common with targeted nucleases increasing the demand for rat models. This protocol details pre-surgical preparation, improved surgical techniques for placing embryos into the oviduct, and post-surgical care of rats to parturition. Direct application of mouse oviduct transfer protocols results in limited success in the rat. By combining techniques from several widely used protocols in the field, increased yield of live pups born to healthy dams is ...

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