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MDA-MB-231

MDA-MB-231

Company: ATCC
Catalog#: CRM-HTB-26
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In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
Author:
Date:
2016-02-20
[Abstract]  Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood vessels (leukocyte mimicry). The shear resistant adhesion is mediated by high affinity interactions between endothelial adhesion molecules and their counter receptor ligand expressed on circulating cells. Thus, weak interaction results in a rapid detachment of circulating cells from endothelium. Despite the critical role of vascular adhesion of cancer cells in hematogenous metastasis, our knowledge regarding this process has been limited due to ... [摘要]  血液转移是转移性癌症死亡的主要原因。循环肿瘤细胞对血流内的血管内皮细胞表面的剪切粘附是细胞外渗和进一步组织侵入的必要步骤。这类似于白细胞用于粘附到发炎血管的方法(白细胞模拟)。剪切抗性粘附由内皮粘附分子和其在循环细胞上表达的其受体配体之间的高亲和力相互作用介导。因此,弱相互作用导致循环细胞从内皮快速分离。尽管癌细胞在血源性转移中的血管粘附的关键作用,我们关于该过程的知识由于难以在体外模拟动态流动条件而受到限制。为了更好地了解癌细胞对内皮的剪切抗性粘附,我们开发了一种用于测量在生理流动条件下循环肿瘤细胞对内皮细胞的剪切抗性粘附的方案,通过适应良好确立的流动粘附测定炎症细胞。该技术可用于评价1)癌细胞的抗剪切粘附能力和2)支持癌细胞粘附所必需的内皮粘附分子(Kang等人,2015)。

Tumor Cell Invasion Assay
Author:
Date:
2012-02-05
[Abstract]  Cell invasion assays have been used to study the interactions between tumor cells and the extracellular matrix (ECM), which not only provides a structural scaffold for the cell, but also contains various biological factors for the survival and growth of the cell. ECM gel contains the basic components of the ECM that provides a structural support for the cell to grow and move. Cells can secrete enzymes that degrade certain components of the ECM to move towards chemoattractants, or to simply establish niches for growth. Metastatic tumor cells often show more invasiveness to the ECM gel due to their higher motility and/or enzymatic activity for degrading ECM components. This protocol describes a tumor cell invasion assay to study the interactions between tumor cells and the ECM. [摘要]  细胞侵袭测定已经用于研究肿瘤细胞和细胞外基质(ECM)之间的相互作用,其不仅为细胞提供结构支架,而且还包含用于细胞存活和生长的各种生物因子。 ECM凝胶含有ECM的基本组分,为细胞生长和移动提供结构支持。 细胞可分泌降解ECM的某些组分以趋向化学引诱物的酶,或简单地建立用于生长的壁龛。 转移性肿瘤细胞通常对ECM凝胶显示更多的侵袭性,这是由于它们对于降解ECM组分的较高的运动性和/或酶活性。 该协议描述了肿瘤细胞侵袭实验,以研究肿瘤细胞和ECM之间的相互作用。

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