| An Acute Mouse Spinal Cord Slice Preparation for Studying Glial Activation ex vivo
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Author:
Date:
2017-01-20
[Abstract] Pathological conditions such as amyotrophic lateral sclerosis, spinal cord injury and chronic pain are characterized by activation of astrocytes and microglia in spinal cord and have been modeled in rodents. In vivo imaging at cellular level in these animal models is limited due to the spinal cord’s highly myelinated funiculi. The preparation of acute slices may offer an alternative and valuable strategy to collect structural and functional information in vitro from dorsal, lateral and ventral regions of spinal cord. Here, we describe a procedure for preparing acute slices from mouse spinal cord (Garré et al., 2016). This preparation should allow further understanding of how glial cells in spinal cord respond acutely to various inflammatory challenges.
[摘要] 病理状况如肌萎缩性侧索硬化，脊髓损伤和慢性疼痛的特征在于脊髓中星形胶质细胞和小胶质细胞的活化，并已在啮齿动物中建模。 在这些动物模型中的细胞水平的体内成像由于脊髓的高度髓鞘化的功能而受到限制。 急性切片的制备可能提供一种替代和有价值的策略，从脊髓的背侧，外侧和腹侧区域体外收集结构和功能信息。 在这里，我们描述了一种从小鼠脊髓制备急性切片的过程（Garréet al。，2016）。 这个准备应该进一步了解脊髓中的神经胶质细胞是如何急剧地反应各种炎症的挑战。
【背景】已经使用小鼠转基因技术来模拟影响脊髓的不同人类病态，其中许多特征为局部胶质激活，神经炎症的一个标志。利用基于激光扫描显微技术的共焦显微镜（White et al。，1987）和双光子显微镜（Denk等，1990），大大提高了对健康和疾病中胶质生物学的认识。 ）以无创的方式可视化活体动物中的细胞结构和亚细胞结构域;例如，表达遗传编码的记者或钙传感器的小鼠已被用于分别形成胶质结构（体细胞和过程）并研究钙动力学和信号传导（Davalos等人，2005; ...
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| Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells
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Author:
Date:
2016-02-20
[Abstract] In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasis in vitro.
[摘要] 在这个协议，我们描述我们修改分离，培养和维持鼠肠干细胞作为隐窝 - 绒毛形成类器官的方法。 从小肠或大肠分离的这些细胞随时间保持自我更新和多向分化潜能。 这为研究人员提供了培养野生型或转化的肠上皮的工具，以及用于体外干细胞组织稳态的稳健试验。
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| Process and Analysis of Kidney Infiltrates by Flow Cytometry from Murine Lupus Nephritis
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Author:
Date:
2012-05-05
[Abstract] Methods for the isolation and characterization of mononuclear phagocytes from the kidneys of mice with SLE are essential to understand the patho-physiology of the disease. Activation of these cells is associated with the onset of clinical disease in mice and infiltration with these cells is associated with poor prognosis in humans.An analysis of the function of these cells should lead to a better understanding of the inflammatory processes that lead to renal impairment in SLE and other renal inflammatory diseases.
[摘要] 从SLE小鼠的肾中分离和表征单核吞噬细胞的方法对于了解疾病的病理生理学是必不可少的。 这些细胞的活化与小鼠中的临床疾病的发作相关，并且这些细胞的浸润与人类的不良预后相关。这些细胞的功能的分析应当导致更好地理解导致肾损伤的炎症过程 在SLE等肾性炎症性疾病中的应用。
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