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Spring Scissors - Angled To Side

春天剪刀 - 倾斜对边
Company: Fine Science Tools
Catalog#: 15006-09
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Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
Author:
Date:
2016-04-05
[Abstract]  We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets et al. (2015). [摘要]  我们在这里提供了详细的协议，用于隔离和培养的主要小鼠感觉神经元。 感觉传入假性极化神经元的细胞体位于沿着脊柱的背根神经节（DRG）中。 解离的小鼠DRG可以通过酶消化解离成单个细胞，以获得小鼠感觉神经元的原代培养物，如Khaminets等人（2015）报道的研究中所进行的。

Isolation and Growth of Adult Mouse Dorsal Root Ganglia Neurons
Author:
Date:
2015-09-20
[Abstract]  Adult dorsal root ganglia neurons are among the few adult neuronal cell types that can be purified and grown relatively easily in dissociated cell culture. Here we describe a procedure for the isolation and growth of dissociated adult mouse DRG neurons using Percoll gradients and a chemically defined medium. These cultures can be used for morphological, biochemical and electrophysiological studies of neuronal growth and function. [摘要]  成年背根神经节神经元是少数成年神经元细胞类型，可以在分离的细胞培养物中相对容易地纯化和生长。 在这里，我们描述了使用Percoll梯度和化学定义的培养基分离和生长分离的成年小鼠DRG神经元的过程。 这些培养物可用于神经元生长和功能的形态学，生物化学和电生理学研究。

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