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Chitin Resin

甲壳素树脂

Company: New England Biolabs
Catalog#: S6651S
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Random DNA Binding Selection Assay (RDSA)
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2015-04-20
[Abstract]  Protein-DNA interaction is a very important cellular process, by which regulation of DNA biological function, usually gene expression, is exerted. The method of random DNA binding selection assay (RDSA) can be used to identify DNA elements bound by proteins with DNA-binding activities. This method is based on the enrichment of the target DNA element by the immobilized recombinant protein on special beads and repeated PCR amplification. [摘要]  蛋白质-DNA相互作用是非常重要的细胞过程,通过其调节DNA生物学功能,通常是基因表达。 随机DNA结合选择测定(RDSA)的方法可以用于鉴定由具有DNA结合活性的蛋白质结合的DNA元件。 该方法基于固定的重组蛋白在特异性珠上的靶DNA元件的富集和重复PCR扩增。

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