| Quantitative Determination of Ascorbate from the Green Alga Chlamydomonas reinhardtii by HPLC
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Author:
Date:
2016-12-20
[Abstract] Ascorbate (Asc, also called vitamin C) is of vital importance to the cellular functions of both animals and plants. During evolution, Asc has become one of the most abundant metabolites in seed plants; however, Asc contents in cyanobacteria, green algae and bryophytes are very low. Here we describe a sensitive and reliable HPLC method for the quantitative determination of cellular Asc content in the green alga Chlamydomonas reinhardtii.
[摘要] 抗坏血酸（Asc，也称为维生素C）对动物和植物的细胞功能至关重要。 在进化过程中，Asc已经成为种子植物中最丰富的代谢物之一; 然而，蓝藻，绿藻和苔藓植物中的Asc含量非常低。 在这里，我们描述了一种灵敏可靠的HPLC方法，用于定量测定绿藻衣藻中细胞Asc含量。
背景 针对高等植物开发了用于确定细胞Asc含量的先前方案，其中细胞Asc的量足够高以用于分析。 这些方案不适用于绿色藻类，因为植物材料的量和细胞Asc的水平通常都受到限制。 因此，需要开发一种灵敏度提高到μM范围来测量绿藻中细胞Asc的新方法。
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| Pot Level Drought Stress Tolerance Assay in Tobacco through Plant Phenotyping and Antioxidant Assay
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Author:
Date:
2015-10-05
[Abstract] Drought is an important abiotic factor which has a huge detrimental impact on crop productivity. Study of plant responses towards drought stress and investigating the mechanism of drought tolerance is crucial for achieving the target of developing drought-tolerant plants. Phenotyping is a cost effective approach which can be adopted to evaluate the severity of drought stress in a plant. Next to phenotyping parameters, biochemical parameters such as the study of antioxidant enzyme activity play significant roles in assessing the extent of drought stress caused injury in a plant. Among the antioxidant enzymes, ascorbate peroxidase is an enzyme which plays a crucial role in drought tolerance in plants. It has been well established that the activity of this enzyme increases under drought ...
[摘要] 干旱是一个重要的非生物因素，对作物生产力有巨大的不利影响。研究植物对干旱胁迫的反应和研究干旱耐受机制对于实现发展耐旱植物的目标是至关重要的。表型是一种成本有效的方法，可用于评价植物中干旱胁迫的严重性。除了表型参数之外，生物化学参数例如抗氧化酶活性的研究在评估植物中干旱胁迫引起的损伤的程度中起重要作用。在抗氧化酶中，抗坏血酸过氧化物酶是在植物的耐旱性中起关键作用的酶。已经确定该酶的活性在干旱胁迫下增加。在这里，我们提出了一个简单和可重复的协议，以调查烟草植物对干旱胁迫通过测量表型参数和抗氧化酶活性的反应。虽然，这些实验已经进行了烟草植物，这个协议可以采用其他作物品种。
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| Measurement of Net High-affinity Urea Uptake in Maize Plants
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Author:
Date:
2015-06-05
[Abstract] Despite its extensive use as a nitrogen fertilizer, the role of urea as a directly accessible nitrogen source for crop plants is still poorly understood. So far, the physiological and molecular aspects of urea acquisition have been investigated only in a few plant species highlighting the importance of a urea transporter in roots, DUR3 (Kojima et al., 2007; Wang et al., 2012; Zanin et al., 2014a). Regarding maize plants, a crop that needs a large amount of urea fertilizer, the capability to take up urea via an inducible and high-affinity transport system has been recently characterized (Zanin et al., 2014a; Zanin et al., 2014b). Here, we described a small-scale protocol suitable for the measurement of urea net high-affinity uptake in roots of ...
[摘要] 尽管其作为氮肥的广泛使用，但是作为直接可及的作物植物氮源的脲的作用仍然知之甚少。 迄今为止，仅在少数植物物种中研究了尿素获取的生理学和分子学方面，突出了根中的尿素转运蛋白DUR3的重要性（Kojima等人，2007; et al。，2012; Zanin et al。，2014a）。 关于玉米植物，需要大量尿素肥料的作物，通过诱导型和高亲和力转运系统摄取尿素的能力最近已被表征（Zanin等人，2014a; Zanin et al。，2014b）。 在这里，我们描述了一个小规模的协议，适合测量尿素净高亲和力摄取完好的玉米植物的根。
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