2,3,5-Triphenyltetrazolium chloride
Company: Sigma-Aldrich
Catalog#: T8877
Product evaluation:
Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells
Author:
Date:
2017-01-20
[Abstract]  This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified using a spectrophotometer. This protocol allows the production of reproducible results in a relatively short time for Sinorhizobium meliloti 1021 cells grown both in exponential and ...

Seed Coat Permeability Test: Tetrazolium Penetration Assay
Author:
Date:
2014-07-05
[Abstract]  Seed coat permeability is important to study as it plays significant roles in seed dormancy, germination, and protection from pathogens. Here we describe a commonly used seed coat permeability test known as the tetrazolium penetration assay with a method to quantify the levels of permeability. Tetrazolium red is a cationic dye that is widely used in seed viability testing. Tetrazolium salts are amphipathic cations, which, after penetrating the dead cells of the seed coat, are reduced to ...

Seed Germination and Viability Test in Tetrazolium (TZ) Assay
Author:
Date:
2013-09-05
[Abstract]  Tetrazolium (TZ) assay is the fast evaluation for seed viability and alternative quick method for seed’s germinability (Porter et al., 1947; Wharton, 1955). All respiring tissues are capable of converting a colourless compound, TZ (2,3,5 triphenyl tetrazolium chloride) to a carmine red coloured water-insoluble formazan by hydrogen transfer reaction catalysed by the cellular dehydrogenases. TZ enters both living and dead cells but only living cells catalyse the formation of formazan ...

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