| Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
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Author:
Date:
2016-09-05
[Abstract] Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for salinity and drought treatments, the soil-culture method in this study was more applicable to studying natural stress conditions.
[摘要] 盐度和干旱是导致红麻产量和质量损失的两个主要因素。 据报道，盐度和干旱可影响10％以上的耕地，导致主要作物平均单产下降50％以上。 因此，了解植物对干旱和盐分的耐受性具有根本重要性，已成为深入研究的重点。 该协议描述了在土壤条件下模拟天然盐度和干旱胁迫的简单和可重复的方案。 即使水培法最常用于盐分和干旱处理，本研究中的土壤培养方法更适用于研究自然胁迫条件。
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| 15N-nitrate Uptake Activity and Root-to-shoot Transport Assay in Rice
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Author:
Date:
2016-08-20
[Abstract] 15N is a nonradioactive heavy isotope of nitrogen, widely used for biochemical and physiological research in plants. For instance, 15N-KNO3 was used as the nitrogen source in plants in order to investigate nitrate uptake activity and transport from roots to shoots (Lin et al., 2008). Here, we describe a detailed pipeline used for labeling living rice (Oryza sativa) plants with 15N-KNO3 and determination of net nitrate uptake and transport activity, and this protocol was proved to be valid in Arabidopsis and rice (Lin et al., 2008; Hu et al., 2015).
[摘要] N是氮的非放射性重同位素，广泛用于植物的生化和生理研究。 例如，使用N-KNO 3作为植物中的氮源，以研究硝酸盐吸收活性和从根到芽的转运（Lin等人 。， 2008）。 在这里，我们描述了用于标记具有 N-KNO 3的活水稻（>稻）植物和测定硝酸盐硝酸盐吸收的详细管道 和转运活性，并且该方案在拟南芥（Arabidopsis）和水稻中被证明是有效的（Lin等人，2008; Hu等人， 2015）。
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| Infection Assays of Tomato and Apple Fruit by the Fungal Pathogen Botrytis cinerea
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Author:
Date:
2014-12-05
[Abstract] Botrytis cinerea (B. cinerea) is an aggressive fungal pathogen that infects more than 200 plant species. Furthermore, the pathogen can attack fruits of some plants, such as tomato and apple. B. cinerea has become one of the model systems in molecular phytopathology because of its economic importance and sophisticated genetic operation methods. Virulence assays are very important in the study of fungal pathogenesis. This protocol details the artificial inoculation procedure of B. cinerea on tomato and apple fruits. It also can be used to analyse the virulence of postharvest fungal pathogens on other fruits, such as pear, peach, jujube and so on.
[摘要] （灰葡萄孢菌（Botrytis cinerea））是侵袭性的真菌病原体，其感染超过200种植物物种。 此外，病原体可攻击一些植物的果实，例如番茄和苹果。 B。 灰霉病已经成为分子植物病理学的模型系统之一，因为其经济重要性和复杂的遗传操作方法。 毒力测定在真菌发病机理的研究中非常重要。 该协议详述了B的人工接种程序。 西红柿和苹果果实上的灰霉菌。 它还可用于分析收获后真菌病原体对其他果实如梨，桃，枣等的毒力。
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