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MicroAmpTM Optical Adhesive Film

MicroAmp ®光学胶粘膜

Company: Thermo Fisher Scientific
Catalog#: 4311971
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Polysome Fractionation to Analyze mRNA Distribution Profiles
Author:
Date:
2017-02-05
[Abstract]  Eukaryotic cells adapt to changes in external or internal signals by precisely modulating the expression of specific gene products. The expression of protein-coding genes is controlled at the transcriptional and post-transcriptional levels. Among the latter steps, the regulation of translation is particularly important in cellular processes that require rapid changes in protein expression patterns. The translational efficiency of mRNAs is altered by RNA-binding proteins (RBPs) and noncoding (nc)RNAs such as microRNAs (Panda et al., 2014a and 2014b; Abdelmohsen et al., 2014). The impact of factors that regulate selective mRNA translation is a critical question in RNA biology. Polyribosome (polysome) fractionation analysis is a powerful method to assess the association of ... [摘要]  真核细胞通过精确调节特定基因产物的表达来适应外部或内部信号的变化。蛋白质编码基因的表达受到转录和转录后水平的控制。在后面的步骤中,翻译的调节在需要蛋白质表达模式快速变化的细胞过程中特别重要。 mRNA的翻译效率由RNA结合蛋白(RBP)和非编码(nc)RNA如微RNA(Panda等人,2014a和2014b; Abdelmohsen等人)改变2014)。调节选择性mRNA翻译的因素的影响是RNA生物学中的一个关键问题。多核糖体(多核糖体)分馏分析是评估核糖体与给定mRNA的关联的有效方法。它提供了关于该mRNA的翻译状态的有价值的信息,这取决于与它们相关联的核糖体的数目,并且鉴定未翻译的mRNA(Panda等人,2016)。与许多核糖体相关的mRNA形成大量的多核糖体,预计将被主动翻译,而与少数或没有核糖体相关的mRNA有可能翻译不佳。总之,多聚糖分馏分析允许直接测定整个转录组水平的翻译效率以及个体mRNA。

背景 ...

The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis
Author:
Date:
2016-10-20
[Abstract]  Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally the growth into macroscopic secondary tumors. This complexity makes metastases difficult to investigate and quantify in animal models. The chick embryo is a unique in vivo model that overcomes many limitations for studying the metastatic process, due to the accessibility of the chorioallantoic membrane (CAM), a well-vascularized extra-embryonic tissue located under the eggshell, that is receptive to the xenografting of mammalian tumor cells, including human. Since the chick ... [摘要]  转移是一个复杂的过程,包括几个步骤:肿瘤进展,血管生成,细胞迁移和入侵,附近血管内渗,循环系统中的生存,外渗,然后归巢到远端组织,微转移的形成,最后生长宏观继发性肿瘤。这种复杂性使得转移瘤难以在动物模型中研究和量化。鸡胚是独特的体内模型,其克服了用于研究转移过程的许多限制,这是由于绒毛尿囊膜(CAM)的可接近性,所述绒毛尿囊膜是位于蛋壳下的良好血管化的胚胎外组织,其接受哺乳动物肿瘤细胞(包括人)的异种移植。由于鸡胚在此阶段是天然免疫缺陷的,所以CAM可以支持肿瘤细胞的植入,并且其中的生长可忠实地重现致癌过程的大多数特征,包括:生长,侵入,血管生成和远端组织的定居(Deryugina和Quigley,2008; Zijlstra等人,2002)。 CAM在癌细胞移植后5-7天内维持快速肿瘤形成。这个特征提供了用于快速研究转移性级联的灌注和定植步骤的独特实验模型。此外,使用定量PCR来检测物种特异性序列,例如Alu ,鸡胚CAM模型可用于监测和定量在远端组织中异种移植的异位肿瘤细胞的存在。因此,鸡胚模型已经证明是用于癌症研究的有价值的工具,特别是用于研究癌症转移中涉及的分子和途径,并分析转移性癌症对潜在疗法的反应(Herrero等人 。,2015; Casar 。,2014)。在这方面,使用快速和定量的自发转移鸡胚胎模型可以提供用于筛选抗癌剂的常规小鼠模型系统的替代方法。

Quantification of HIV RNA and Human Herpesvirus DNA in Seminal Plasma
Author:
Date:
2015-05-05
[Abstract]  Multiple viruses can co-infect the genital tract, modifying the immunologic and virologic milieu and possibly playing a role in viral transmission and pathogenesis. The aim of our studies has been to understand the complex relationships between HIV-1 RNA, and multiple human herpesviruses known to frequently replicate in the genital tract of HIV-infected men (i.e. cytomegalovirus [CMV], Epstein Bar virus [EBV], herpes simplex virus [HSV] types 1 and 2, and human herpesviruses [HHV] 6, 7 and 8) (Gianella et al., 2013a; Gianella et al., 2013b; Gianella et al., 2013c; Gianella et al., 2014). This protocol was designed to collect and process male genital secretion (GS), and to isolate and further quantify HIV RNA and DNA of seven HHV from seminal ... [摘要]  多种病毒可以共感染生殖道,修改免疫学和病毒学环境,并可能在病毒传播和发病中起作用。 我们的研究的目的是了解HIV-1 RNA和已知在HIV感染的男性生殖道中经常复制的多种人疱疹病毒之间的复杂关系(即巨细胞病毒[CMV],Epstein 条形病毒[EBV],单纯疱疹病毒[HSV] 1型和2型,以及人疱疹病毒[HHV] 6,7和8)(Gianella等人,2013a; Gianella等人 。,2013b; Gianella 等人,2013c; Gianella ,2014)。 该方案设计用于收集和处理男性生殖器分泌(GS),并使用定量实时PCR技术从精浆中分离并进一步定量7个HHV的HIV RNA和DNA。

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