Company: Sigma-Aldrich
Catalog#: L9504
Product evaluation:
Determination of Luciferase Activity in Arabidopsis seedling
[Abstract]  There are different direct and indirect methods available to study gene expression in plant systems. In this protocol we describe a modified expression assay using transgenic plants expressing the luciferase gene under the control of phosphate transporter PHT1;1 promoter. This assay was originally optimized for analyzing the repression of PHT1;1 promoter in response to arsenate As (V) which can be adapted to study the kinetics of transcriptional regulation of any gene in response to biotic or ...

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