Collagenase from Clostridium histolyticum
Company: Sigma-Aldrich
Catalog#: C6885
Product evaluation:
Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes
Author:
Date:
2017-04-20
[Abstract]  Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia. The protocol outlined herein has been used to examine the response of ion channels expressed in Xenopus oocytes to LSS (Hoger et al., 2002; Carattino et al., 2004; Shi et ...

Glioma Associated Stem Cells (GASCs) Isolation and Culture
Author:
Date:
2015-05-05
[Abstract]  Glioma Associated Stem Cells (GASCs) represent a population of non-tumorigenic multipotent stem cells hosted in the microenvironment of human gliomas. In vitro, these cells are able, through the release of exosomes, to increase the biological aggressiveness of glioma-initiating cells. The clinical importance of this finding is supported by the strong prognostic value associated with the GASCs surface immunophenotype thus suggesting that this patient-based approach can provide a ...

Preparation of Adult Mouse Muscle Stem Cells
Author:
Date:
2013-12-20
[Abstract]  Muscle stem cells are adult stem cells responsible for muscle development, growth and regeneration. Current knowledge suggests those cells are heterogeneous population shared their position between the sarcolemma and basal lamina of muscle fibers. This protocol describes the technique to dissociate and collect the stem cells from skeletal muscle of adult mice, and separate them from other cells found in muscle (e.g. fat, connective tissue). To purify and preserve those myofiber ...

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