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Author:
Date:
2014-05-20
[Abstract] The aim of this experiment is to study mitochondrial functional status in Arabidopsis embryo sacs using the membrane potential indicator JC-1. Changes in the membrane potential are presumed to be due to the opening of the mitochondrial permeability transition pore (MPTP), allowing passage of ions and small molecules. The resulting equilibrium of ions leads in turn to the decoupling of the respiratory chain and the release of cytochrome c into the cytosol, a distinctive feature of the early stages of programmed cell death.
JC-1 is a lipophilic dye that can selectively enter into mitochondria and reversibly change color from green to red as the membrane potential increases. In healthy cells with high mitochondrial potential, JC-1 spontaneously forms complexes with ...
[摘要] 该实验的目的是使用膜电位指示剂JC-1研究拟南芥胚胎囊中的线粒体功能状态。假定膜电位的变化是由于线粒体通透性转换孔（MPTP）的打开，允许离子和小分子通过。所产生的离子平衡又导致呼吸链的解耦和细胞色素c释放到细胞溶质中，这是程序性细胞死亡的早期阶段的显着特征.JC-1是一种亲脂性染料，其可以选择性地进入线粒体并随着膜电位增加可逆地将颜色从绿色改变为红色。在具有高线粒体电位的健康细胞中，JC-1自发形成具有强烈红色荧光的复合物。另一方面，在具有低线粒体电位的线粒体中，JC-1保持单体形式，其仅显示绿色荧光（Martin等人，2013; Hauser等人， em，2006）。
此协议可用于孤立的线粒体，以及各种细胞类型和植物和其他生物体的不同组织。
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