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Company: Phenomenex
Catalog#: C18-E
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Metabolite and Fatty Acid Analysis of Yeast Cells and Culture Supernatants
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2014-09-05
[Abstract]  Metabolite and fatty acid analysis play important roles in evaluating the metabolic state of microorganisms. To examine the growth state and metabolism response of cells to environmental stress or genetic modification, intracellular and extracellular metabolites including fatty acids are usually analyzed to help understand the cellular biochemical changes in microorganisms. In this protocol, gas chromatography-mass spectrometry based analysis was employed to investigate the fatty acids and other metabolites in yeast cells. [摘要]  代谢物和脂肪酸分析在评估微生物的代谢状态中起重要作用。 为了检查细胞对环境胁迫或遗传修饰的生长状态和代谢反应,通常分析细胞内和细胞外代谢物(包括脂肪酸)以帮助理解微生物中的细胞生物化学变化。 在本协议中,采用基于气相色谱 - 质谱法的分析来研究酵母细胞中的脂肪酸和其他代谢物。

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