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3,3'-Diaminobenzidine

3,3''-二氨基联苯胺

Company: Sigma-Aldrich
Catalog#: D8001
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Detection of Reactive Oxygen Species in Oryza sativa L. (Rice)
Author:
Date:
2016-12-20
[Abstract]  Superoxide ions (O2-) and hydrogen peroxide (H2O2) are the reactive oxygen species (ROS) that play a significant role in regulation of many plant processes. The level of O2- ions is determined qualitatively using nitrobluetetrazolium (NBT) assay while the H2O2 is qualitatively estimated using 3,3-diaminobenzidine (DAB) and 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) assay. Further the aqueous content of H2O2 is estimated quantitatively using ferrous oxidation-xylenol orange (FOX) assay. [摘要]  超氧化物离子(O 2 - )和过氧化氢(H 2 O 2 O 2)是起重要作用的活性氧(ROS)在许多植物过程的调节。使用硝基四氮唑(NBT)测定定性地测定O 2 - 离子的水平,同时使用3,3 - 二氨基联苯胺(DAB)和2',7'-二氯二氢荧光素二乙酸酯(H 2 N 2 DCFDA)测定。此外,使用亚铁氧化 - 二甲苯酚橙(FOX)测定定量地估计H 2 O 2 O 2的含水量。

背景 超氧化物离子和过氧化氢是在许多情况下起主要作用的活性氧分子涉及植物生长发育的过程包括非生物胁迫耐受性。为了更好地了解ROS对这些过程的调节,对不同类型ROS的定性和定量估计具有重要意义。通过由NADPH氧化酶系统介导的电子从NADPH转移到氧(O 2),产生O 2。使用NBT测定法在水稻幼苗中估计这些离子,其基于通过O将黄色NBT还原成深蓝色不溶性甲the的原理(Kaur等人,2016)。
  H 2是另一种作为调节不同植物过程的重要信号分子的活性氧分子。在使用DAB和H 2 DCFDA测定的水稻幼苗中定性地估计H 2 O 2 O 2的含量(Kaur等人,2016)。 DAB测定是基于DAB与H 2 O 2 O 2的反应形成深棕色聚合产物的原理,而H 2 N 2 ...

Histochemical Detection of Superoxide and H2O2 Accumulation in Brassica juncea Seedlings
Author:
Date:
2014-04-20
[Abstract]  Plant cells continually produce reactive oxygen species (ROS) as a by-product of aerobic metabolism. Increased production of ROS occurs under unfavorable conditions imposed by various abiotic and biotic factors. Accumulation of ROS is damaging to various cellular components and macromolecules including plasma membrane, nucleic acids, and proteins and eventually leads to cell death. In this protocol, we describe the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2-) anion, two of the most important ROS, in Brassica juncea seedlings by using 3,3ʹ-Diaminobenzidine (DAB) and Nitrotetrazolium blue chloride (NBT) as the chromogenic substrate. DAB is oxidized by H2O2 in the presence of peroxidases ... [摘要]  植物细胞不断产生活性氧(ROS)作为有氧代谢的副产物。增加的ROS的产生发生在由各种非生物和生物因素施加的不利条件下。 ROS的积累损害各种细胞组分和大分子,包括质膜,核酸和蛋白质,并最终导致细胞死亡。在该方案中,我们描述了过氧化氢(H 2 O 2 O 2)和超氧化物(O 2 2 O 2)的组织化学检测,通过使用3,3'-二氨基联苯胺(DAB)和硝基四唑氯化蓝(NBT)作为显色底物,在芥菜籽苗中培养两种最重要的ROS。 DAB在过氧化物酶存在下被H 2 O 2 O 2氧化并产生红棕色沉淀。 NBT与O 2反应以形成深蓝色不溶性甲an化合物。该方案可用于其他植物物种和不同的植物组织。

DAB Staining and Visualization of Hydrogen Peroxide in Wheat Leaves
Author:
Date:
2012-12-20
[Abstract]  The production of hydrogen peroxide (H2O2) has been recognized as an important feature of plant cells that undergo programmed cell death (PCD) during host-pathogen interaction. Thordal-Christensen et al. (1997) first described a method using chemical 3,3-diaminobenzidine (DAB) to detect the presence and distribution of H2O2 in barley leaves challenged by the powdery mildew fungus (Thordal-Christensen et al., 1997). Since then, this method has been adapted to many other plant species for in situ detection of H2O2. Here, we describe a modified protocol to stain and visualize H2O2 production in wheat leaves during infection by the necrotrophic fungus, Stagonospora ... [摘要]  过氧化氢(H 2 O 2 O 2)的产生已被认为是在宿主 - 病原体相互作用期间经历程序性细胞死亡(PCD)的植物细胞的重要特征。 Thordal-Christensen等人(1997)首先描述了使用化学3,3-二氨基联苯胺(DAB)检测H 2 O 2基团的存在和分布的方法, (Thordal-Christensen等人,1997)攻击的大麦叶中的抗性。 从那时起,该方法已经适应于许多其它植物物种用于原位检测H 2 O 2 O 2。 在这里,我们描述了在感染期间通过坏死性真菌,角叉状瘤或由产生的坏死营养效应物的浸润来染色和可视化小麦叶中H 2 O 2 O 2产生的修饰的方案 真菌。 该方法的简短版本已经在(Liu等人2012年)中报道。

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