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Odyssey infrared EMSA kit

Odyssey红外线EMSA套件

Company: LI-COR
Catalog#: 829-07910
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Electrophoresis Mobility Shift Assay
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2014-04-05
[Abstract]  Protein (transcription factors and/or transcription cofactors)-binding to DNA is a critical event in regulation of transcription. Electrophoresis Mobility Shift Assay (EMSA), also known as gel shift assay, is a useful tool to detect protein- or protein complex-DNA/RNA interaction and to evaluate DNA binding specificity of transcription factors in vitro. Here we describe a simple method for EMSA with fluorescent dye-bound oligo DNA probes and recombinant protein expressed in bacterial cells. Using fluorescent dye instead of radioisotope enables easy handling and long-term storage of labelled-probes without reduction of detection sensitivity. [摘要]  与DNA结合的蛋白质(转录因子和/或转录辅因子)是调节转录的关键事件。 电泳迁移率变动分析(EMSA),也称为凝胶移位分析,是检测蛋白质或蛋白质复合物-DNA/RNA相互作用和评价转录因子的体外DNA结合特异性的有用工具。 在这里我们介绍一种简单的方法与荧光染料绑定寡DNA探针和表达在细菌细胞中的重组蛋白的EMSA。 使用荧光染料代替放射性同位素使得容易处理和长期储存标记的探针而不降低检测灵敏度。

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