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Author:
Date:
2016-08-05
[Abstract] Mesenchymal stem cells (MSCs) are widespread in adult organisms and involved in tissue maintenance and repair as well as in the regulation of hematopoiesis and immunologic responses. As cell adhesion play important roles in cell interactions and signaling, to thoroughly evaluate the adhesion ability of MSCs is of vital importance to clarify the mechanism of self-renewal, proliferation, activation and migration of MSCs in different microenvironments. Based on the method by Siler et al., 2000, we revised the protocol in order to provide details on how to evaluate the adhesion ability of MSCs from bone marrow (BMSCs) on extracellular matrix (ECM) protein laminins. The current protocol can also be easily translated to MSCs with other treatments or ECMs such as collagens, fibronectin, etc ...
[摘要] 间充质干细胞（MSC）在成年生物体中广泛存在并参与组织维持和修复以及造血作用和免疫反应的调节。 由于细胞粘附在细胞相互作用和信号转导中发挥重要作用，彻底评估MSC的粘附能力对于阐明MSC在不同微环境中的自我更新，增殖，活化和迁移的机制至关重要。 基于Siler等人的方法（2000），我们修订方案以提供关于如何评价来自骨髓（BMSCs）的MSC对细胞外基质（ECM）蛋白的粘附能力的细节 层粘连蛋白。 目前的方案也可以很容易地翻译成MSC与其他治疗或ECM，如胶原，纤连蛋白，等。
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