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PrecisionGlideTM Needles

PrecisionGlide TM

Company: BD
Catalog#: 305156
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In vitro DNA Polymerization Activity Assay Using Cell-free Extracts
Author:
Date:
2014-08-20
[Abstract]  This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher temperature (22 °C) can be attributed to the reduced rate of DNA replication at low temperature. Here we describe a protocol which we have used to quantify the in vitro DNA polymerization of cell extracts at two different temperatures. [摘要]  该方案已经设计用于测量南极细菌假单胞菌syrinagae Lz4W的粗细胞提取物中的体外 DNA聚合活性。 该细菌可以在4℃下生长,在22℃以最佳生长速率生长。 在低温(4℃)下观察到的细菌与较高温度(22℃)相比的缓慢生长速率可以归因于在低温下DNA复制的速率降低。 在这里我们描述了一个协议,我们已经用来量化的体外细胞提取物在两个不同的温度下的DNA聚合。

Yeast Transcription Factor Chromatin Immunoprecipitation
Author:
Date:
2011-07-05
[Abstract]  This ChIP protocol was developed and improved over the years by various researchers in the Snyder lab, Stanford University, especially Anthony Borneman and Christopher Yellman. I have used this method to successfully map the genome-wide binding of transcription factors Ste12. The ChIPed DNA is suitable for downstream analysis using PCR, microarray or sequencing. [摘要]  这个ChIP协议是由斯坦福大学斯奈德实验室,特别是安东尼博恩曼和克里斯托弗·耶尔曼的研究人员多年来开发和改进的。 我已经使用这种方法成功映射转录因子Ste12的全基因组结合。 ChIPed DNA适用于使用PCR,微阵列或测序的下游分析。

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