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Author:
Date:
2015-09-05
[Abstract] We recently demonstrated the presence of a quinone detoxification pathway present in Firmicutes. It is based on two enzyme activities, namely a hydroquinone dioxygenase, YaiA, described here, and a hydroquinone reductase, YaiB, described in a separate protocol. In Lactococcus lactis (L. lactis), these enzymes are encoded by the yahCD-yaiAB operon. The operon is induced by copper to prevent the synergistic toxicity of quinones and copper. The hydroquinone dioxygenase, YaiA, converts hydroquinones to 4-hydroxymuconic semialdehyde, using molecular oxygen as oxidant according to the reaction: hydroquinone + O2 → 4-hydroxymuconic semialdehyde + H+ We here describe two methods for measurements for hydroquinone dioxygenase activity, based on ...
[摘要] 我们最近展示了存在于Firmicutes中的醌解毒途径。 它基于两种酶活性，即本文所述的氢醌双加氧酶，YaiA和在单独方案中描述的氢醌还原酶，YaiB。 在乳酸乳球菌（乳酸乳杆菌）中，这些酶由yahCD-yaiAB 操纵子编码。 操纵子由铜诱导以防止醌和铜的协同毒性。 根据以下反应，氢醌双加氧酶YaiA使用分子氧作为氧化剂将氢醌转化为4-羟基粘康酸半醛：氢醌+ O 2→4-羟基粘康酸半醛+ H + 我们在这里描述了用于测量氢醌双加氧酶活性的两种方法，基于用氧电极测量的氧消耗和4-羟基粘康酸半醛的分光光度检测。 两种测定用粗细胞提取物进行。
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