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MEM α, nucleosides, no phenol red

MEM α, nucleosides, no phenol red

Company: Thermo Fisher Scientific
Catalog#: 41061029
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Limiting Dilution Assays to Determine Frequencies of Lymphohematopoietic Progenitors
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2014-02-20
[Abstract]  This protocol is useful to determine the frequencies of lymphohematopoietic progenitors in tested samples. To effectively support the growth and differentiation of primitive lymphohematopoietic progenitors, complex signals from stromal cells are important. Several stromal cell lines are known to support both lymphoid and myeloid cells simultaneously in mouse. In this protocol, we introduce two stromal co-culture systems for murine lymphohematopoietic progenitors and their application for limiting dilution assays. [摘要]  这个协议是有用的确定淋巴造血祖细胞在测试样品中的频率。 为了有效支持原始淋巴造血祖细胞的生长和分化,来自基质细胞的复合信号是重要的。 已知几种基质细胞系在小鼠中同时支持淋巴和骨髓细胞。 在这个协议,我们介绍两个基质共培养系统鼠淋巴造血祖细胞及其应用限制稀释测定。

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