{{ 'Home' | translate }} {{'About us' | translate}} {{'Sponsors' | translate}}
 
{{'Search' | translate}}
 

SnakeskinTM membrane

SnakeSkin 透析管，3.5K MWCO，22mm
Company: Thermo Fisher Scientific
Catalog#: 68035
Bio-protocol()
Company-protocol()
Other protocol()
In vitro Analysis of Ubiquitin-like Protein Modification in Archaea
Author:
Date:
2018-05-20
[Abstract]  The ubiquitin-like (Ubl) protein is widely distributed in Archaea and involved in many cellular pathways. A well-established method to reconstitute archaeal Ubl protein conjugation in vitro is important to better understand the process of archaeal Ubl protein modification. This protocol describes the in vitro reconstitution of Ubl protein modification and following analysis of this modification in Haloferax volcanii, a halophilic archaeon serving as the model organism. [摘要]  泛素样（Ubl）蛋白广泛分布于古细菌中并参与许多细胞途径。 为了更好地理解古细菌Ub1蛋白质修饰的过程，重建体外古细菌Ubl蛋白质缀合物的完善方法是很重要的。 该协议描述了Ubl蛋白质修饰的体外重建以及在作为模型生物的嗜盐古细菌Haloferax volcanii 中对这种修饰进行分析。

【背景】泛素（Ub）与靶蛋白共价连接的过程被称为泛素化，其控制真核细胞中大量的细胞过程（Glickman和Ciechanover，2002; Komander和Rape，2012）。遍在蛋白化由一系列酶（包括Ub激活酶（E1），Ub结合酶（E2s）和Ub连接酶（E3s））催化。泛素化的体外重建是确定酶之间或E3与蛋白质底物之间特异性的有用测定法（Zhao等人，2012）。在古细菌中，Ubl蛋白SAMP采用Ub折叠，并且与E1样酶UbaA催化的蛋白靶标异肽连接[Maupin-Furlow，（2014）综述]。尽管E1同系物在古细菌中广泛存在，但基于一级序列比较，在大多数古细菌中未预测经典E2或E3酶。我们最近对Haloferax volcanii的研究表明甲硫氨酸亚砜还原酶A（MsrA）是Ubl蛋白质修饰（sampylation）与UbaA一起在体内温和的氧化条件下和< （体外）（fu="">

Antibody Purification from Western Blotting
Author:
Date:
2012-03-20
[Abstract]  This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in case antigen protein solubility is limited. Disadvantage is that possible antibodies that recognize certain 3D structure in solution of antigens might not be purified using such a method. Regarding this issue, the flow through is recommended to be kept and can be used for other purification methods with folded antigens. [摘要]  该方案描述了从固定在western印迹膜上的变性抗原的血清中纯化抗体的方法。 优点包括（1）快速和容易; （2）用变性形式的抗原纯化抗体在抗原蛋白溶解度有限的情况下允许高产率。 缺点是在抗原溶液中识别某些3D结构的可能的抗体可能不能使用这种方法纯化。 关于这个问题，推荐保持流通，并且可以用于具有折叠抗原的其它纯化方法。

Comments



twitter facebook linkedin
{{'About us' | translate}}
 
{{'Contact US' | translate}}

©  Bio-protocol LLC.
Terms of Service | Copyright IP Policy