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Author:
Date:
2015-08-20
[Abstract] Glucosinolates (GLS) are secondary metabolites mainly found in plants belonging to the Brassicaceae family, including also horseradish (Armoracia rusticana G. Gaertn., B. Mey. & Scherb), a popular spice with a characteristic pungent flavor due to the abundance of GLS. Such compounds exhibit antibacterial, antifungal, and insecticidal activities, as well as human health properties. Therefore, it is very important to have a full understanding of their levels and profiles in plants. However, the characterization of GLS from horseradish crude extracts is a tough task, due to the complexity of the vegetal matrix and the occurrence of many GLS in trace amounts. Here we describe two alternative effective and rapid methods for GLS characterization in horseradish plants: Liquid chromatography ...
[摘要] 硫代葡萄糖苷（GLS）是次要代谢物，主要在属于十字花科的植物中发现，包括辣根（Armoracia rusticana G.Gaertn，B.Mey。&Scherb），由于GLS丰度而具有特征刺激性风味 。 这种化合物表现出抗菌，抗真菌和杀虫活性以及人类健康性质。 因此，对其在植物中的水平和谱的完全理解是非常重要的。 然而，由于植物基质的复杂性和痕量的许多GLS的出现，来自辣根粗提取物的GLS的表征是艰难的任务。 在这里我们描述两种替代的有效和快速的方法在辣根植物的GLS表征：液相色谱耦合高分辨率质谱（LC-MS）测定完整的GLS和HPLC-UV测定脱硫-GLS。
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