| An HPLC-based Method to Quantify Coronatine Production by Bacteria
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Author:
Date:
2017-03-05
[Abstract] Coronatine is a polyketide phytotoxin produced by several pathovars of the plant pathogenic bacterium Pseudomonas syringae. It is one of the most important virulence factors determining the success of bacterial pathogenesis in the plant at both epiphytic and endophytic stages of the disease cycle. This protocol describes an optimized procedure to culture bacterial cells for coronatine production and to quantify the amount of coronatine secreted in the culture medium using an HPLC-based method.
[摘要] 冠心病是由植物病原菌的几种病原体产生的聚酮化合物植物毒素。它是在疾病周期的附生和内生期阶段确定植物细菌发病机制成功的最重要的毒力因素之一。该方案描述了用于培养用于冠状病毒产生的细菌细胞的优化方法，并且使用基于HPLC的方法定量培养基中分泌的冠状动脉的量。
背景 Coronatine（COR）是有效的细菌植物毒素，是植物激素茉莉酮酸-L异亮氨酸（JA-Ile）的分子模拟物。因此，COR激活茉莉酸（JA）信号传导，诱导JA响应基因，并拮抗免疫信号水杨酸的作用。 COR由两种成分组成：冠心病（CFA）和冠状氨酸（CMA）。编码CMA和CFA生物合成的基因在细菌中不是组成型表达的。相反，当细菌在诱导培养基中生长时，这些基因在植物叶片表面或植物体内或/或体外诱导（Palmer和Bender，1993; Panchal等等，2016）。本文介绍了从Panchal等人改编的方法。 （2016），以确定细菌产生冠心病的能力，可用作毒性指征。
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| Glucosinolates Determination in Tissues of Horseradish Plant
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Author:
Date:
2015-08-20
[Abstract] Glucosinolates (GLS) are secondary metabolites mainly found in plants belonging to the Brassicaceae family, including also horseradish (Armoracia rusticana G. Gaertn., B. Mey. & Scherb), a popular spice with a characteristic pungent flavor due to the abundance of GLS. Such compounds exhibit antibacterial, antifungal, and insecticidal activities, as well as human health properties. Therefore, it is very important to have a full understanding of their levels and profiles in plants. However, the characterization of GLS from horseradish crude extracts is a tough task, due to the complexity of the vegetal matrix and the occurrence of many GLS in trace amounts. Here we describe two alternative effective and rapid methods for GLS characterization in horseradish plants: Liquid chromatography ...
[摘要] 硫代葡萄糖苷（GLS）是次要代谢物，主要在属于十字花科的植物中发现，包括辣根（Armoracia rusticana G.Gaertn，B.Mey。&Scherb），由于GLS丰度而具有特征刺激性风味 。 这种化合物表现出抗菌，抗真菌和杀虫活性以及人类健康性质。 因此，对其在植物中的水平和谱的完全理解是非常重要的。 然而，由于植物基质的复杂性和痕量的许多GLS的出现，来自辣根粗提取物的GLS的表征是艰难的任务。 在这里我们描述两种替代的有效和快速的方法在辣根植物的GLS表征：液相色谱耦合高分辨率质谱（LC-MS）测定完整的GLS和HPLC-UV测定脱硫-GLS。
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| An Evaluation of Cellulose Degradation Affected by Dutch Elm Disease
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Author:
Date:
2015-07-20
[Abstract] The pathogenic fungus Ophiostoma novo-ulmi spreads within the secondary xylem vessels of infected elm trees, causing the formation of vessel plugs due to tyloses and gels, which ultimately result in Dutch elm disease. Foliage discoloration, wilting and falling from the tree are typical external leaf symptoms of the disease followed by the subsequent death of sensitive trees. Cellulolytic enzymes produced by the fungus are responsible for the degradation of medium molecular weight macromolecules of cellulose, resulting in the occurrence of secondary cell wall ruptures and cracks in the vessels but rarely in the fibers (Ďurkovič et al., 2014). The goal of this procedure is to evaluate the extent of cellulose degradation by a highly aggressive strain of O. novo-ulmi ...
[摘要] 病原真菌新孢子虫在受感染的榆树的次生木质部导管内扩散，导致由于菌丝和凝胶而形成血管栓塞，最终导致荷兰榆树病。叶子变色，枯萎和从树上掉下是典型的外部叶症状的疾病，随后是敏感树的死亡。由真菌产生的纤维素分解酶负责纤维素的中等分子量大分子的降解，导致在血管中发生继发性细胞壁破裂和裂纹，但是在纤维中很少发生破裂（Jurkovic等人， ，2014）。该程序的目的是通过高侵蚀性的O应变评价纤维素降解的程度。 novo-ulmi ssp。 美国× novo-ulmi 。尺寸排阻色谱法（SEC）比较了感染的和未感染的榆树之间的纤维素的分子量分布，并揭示了纤维素的大分子性状的变化，包括分子量，聚合度和多分散指数。 13 C魔幻角旋转核磁共振（ 13 C MAS NMR）光谱有助于鉴定和定量由于降解而导致的结晶和非结晶纤维素区域的损失。本文所述的方法也可以容易地用于感染各种纤维素降解真菌的其它木本植物。
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