BLEOCINTM Antibiotic, Streptomyces verticillus, Cell Culture-Tested
Company: EMD Millipore
Catalog#: 203408
Product evaluation:
DNA Damage Sensitivity Assays with Arabidopsis Seedlings
Author:
Date:
2014-04-05
[Abstract]  We describe fast and reproducible sensitivity assays to quantify the response of Arabidopsis seedlings of different genotypes to a wide range of DNA damaging agents. We apply (1) γ-irradiation, which produces DNA breaks, (2) bleocin, a radiomimetic drug, (3) mitomycin C, a DNA intrastrand cross-linker, (4) hydroxyurea, an inhibitor of DNA synthesis and (5) UV-C, which causes mainly photoproducts. The “true leaf assay” and the “UV resistance assay” are based on easily determined ...

Measuring Homologous Recombination Frequency in Arabidopsis Seedlings
Author:
Date:
2014-04-05
[Abstract]  Somatic homologous recombination (SHR) is a major pathway of DNA double-strand break (DSB) repair, in which intact homologous regions are used as a template for the removal of lesions. Its frequency in plants is generally low, as most DSB are removed by non-homologous mechanisms in higher eukaryotes. Nevertheless, SHR frequency has been shown to increase in response to various chemical and physical agents that cause DNA damage and/or alter genome stability (reviewed in March-Díaz and Reyes, ...

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