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Author:
Date:
2016-10-05
[Abstract] In plant cells, genomic DNA exists in three organelles: the nucleus, chloroplast, and mitochondrion. Genomic DNA can be damaged by endogenous and exogenous factors, but the damaged DNA can be repaired by DNA repair systems. To quantify the extent of their repair activity of on individual genomic DNA, a PCR-based assay utilizing long amplicons is valuable for evaluable. This assay is based on the inhibitory effects of methyl methanesulfonate (MMS)-induced DNA damage on the amplicons. This assay is useful for assessing DNA double-strand repair pathways, such as homologous recombination repair, as it detects DNA double-strand breaks produced by MMS in vivo.
[摘要] 在植物细胞中，基因组DNA存在于三个细胞器中：核，叶绿体和线粒体。基因组DNA可以被内源和外源因子损伤，但损伤的DNA可以通过DNA修复系统修复。为了量化其对单个基因组DNA的修复活性的程度，使用长扩增子的基于PCR的测定对于可评价是有价值的。该测定基于甲磺酸甲酯（MMS）诱导的DNA损伤对扩增子的抑制作用。该测定可用于评估DNA双链修复途径，例如同源重组修复，因为其检测由MMS在体内产生的DNA双链断裂。
[背景] 基因组DNA损伤的定量可用于分析DNA修复机制。该测定利用实时PCR定量核，叶绿体和线粒体DNA拷贝数以用于长PCR产物的标准化，与由Hunter等人先前的方案相比提供更准确的定量。 （2010）。
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Author:
Date:
2016-08-20
[Abstract] Establishing a reservoir of polymorphic markers is an important key for marker-assisted breeding. Many crops are still lack of such genomic infrastructure. Single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) are useful as markers because they are widespread over the genome and many technologies were developed for high throughput genotyping. We present here a pipeline for developing a reservoir of SNP and SSR markers for Mangifera indica L. as an example for fruit tree crops having no genomic information available. Our pipeline includes de novo assembly of reference transcriptome with MIRA and CAP3 based on reads produced by 454-GS FLX technology; Polymorphic loci discovery by alignment of Illumina resequencing to the transcriptome reference; ...
[摘要] 建立多态性标记的储库是标记辅助育种的重要关键。许多作物仍然缺乏这样的基因组学基础设施。单核苷酸多态性（SNP）和简单序列重复（SSR）可用作标记，因为它们在基因组上广泛存在，并且开发了许多技术用于高通量基因分型。我们在这里提出了用于开发Mangifera indica的SNP和SSR标记库的管道，作为没有基因组信息的果树作物的实例。我们的管道包括基于由454-GS FLX技术产生的读数的具有MIRA和CAP3的参考转录组的 de novo 装配;通过Illumina重排序到转录组参考的比对的多态性基因座发现;通过用Fluidigm技术进行基因分型，鉴定整个种质资源集合中多态的基因座子集。 [背景] ...
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