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Blasticidine S hydrochloride


Company: Sigma-Aldrich
Catalog#: 15205
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IFN-α/β Detection Assay Using Sensor Cell Lines
[Abstract]  Type I interferons (IFN-α/β) play an important role in host resistance to viral infections. Signaling through the JAK-STAT pathway, IFN-α/β stimulates response elements (ISRE) in the promoters of ISG to regulate their expression (reviewed in Reference 2). This method was adapted from InvivoGen to specifically detect and quantify IFN-α/β secreted in response to virus infection. HEK-Blue™ IFN-α/β cells were generated by stably introducing the human STAT2 and IRF9 genes into HEK293 cells to obtain a fully active type I IFN signaling pathway. The activation of this pathway is made detectable by the addition of a reporter gene expressing a secreted embryonic alkaline phosphatase (SEAP) under the control of the ISG54 promoter. ISG54 is a well-known ISG activated through an ISRE-dependent ... [摘要]  I型干扰素(IFN-α/β)在宿主对病毒感染的抗性中起重要作用。 通过JAK-STAT途径的信号传导,IFN-α/β刺激ISG启动子中的反应元件(ISRE)以调节它们的表达(参见参考文献2)。 此方法改编自InvivoGen以特异性检测和量化响应于病毒感染分泌的IFN-α/β。 通过将人STAT2和IRF9基因稳定地引入HEK293细胞中以产生完全活性的I型IFN信号传导途径,产生HEK-Blue TM IFN-α/β细胞。 通过加入在ISG54启动子控制下表达分泌性胚胎碱性磷酸酶(SEAP)的报告基因,可以检测该途径的活化。 ISG54是通过I型IFN的ISRE依赖性机制激活的公知的ISG