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Author:
Date:
2016-02-05
[Abstract] In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). Although encouraging results have been obtained in experimental models, the efficiency of cardiac regeneration is very poor and one of the major barriers to progress in the area of cell therapy for damaged heart is represented by the limited capacity of cells to differentiate into mature cardiomyocytes (CMC) (Laflamme and Murry, 2011). Cell manipulation and transfection represent versatile tools in this context (Melo et al., 2005; Dzau et al., 2005). Murine P19 embryonal carcinoma cells are a well-established cell line capable of ...
[摘要] 近年来，已经提出利用干细胞治疗来再生心脏组织作为治疗心脏损伤的可能策略（Gnecchi等人，2012，Aguirre等人， >，2013; Sanganalmath和Bolli，2013）。虽然在实验模型中已经获得令人鼓舞的结果，但是心脏再生的效率非常差，并且在损伤的心脏的细胞治疗领域中进展的主要障碍之一是细胞分化成成熟心肌细胞的能力有限（CMC ）（Laflamme和Murry，2011）。细胞操作和转染在本文中代表多种工具（Melo等人，2005; Dzau等人，2005）。鼠P19胚胎癌细胞是能够在体外分化成自发性搏动CMC的良好建立的细胞系。这种细胞系统具有有限的细胞培养要求，协议重现性和易于摄取和随后的异位遗传材料的表达，使其理想的心脏分化过程的研究。 P19细胞已经成功地用于获得对CMC分化的早期分子过程的重要见解（van der Heyden和Defize，2003; van der ...
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