GelPilot DNA Loading Dye, 5x
Company: QIAGEN
Catalog#: 239901
Product evaluation:
Oral Microbiome Characterization in Murine Models
Author:
Date:
2017-12-20
[Abstract]  The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe murine oral microbiome sampling procedures, processing of low biomass samples and subsequent microbiome characterization based on 16S rRNA gene ...

Determination of Mutation Frequency During Viral DNA Replication
Author:
Date:
2014-03-20
[Abstract]  This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. In the original Carrascosa et al. (1982), the protocol was carried out with two cloned viruses, BA71Vc (a purified clone from BA71V wild type strain) and vΔpolX (lacking the reparative polymerase, pol X, gene), and two different cell types ...

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