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Author:
Date:
2020-03-05
[Abstract] The plant cell wall is a complex network of polysaccharides and proteins that provides strength and structural integrity to plant cells, as well as playing a vital role in growth, development, and defense response. Cell wall polysaccharides can be broadly grouped into three categories: cellulose, pectins, and hemicelluloses. Dynamic interactions between polysaccharides and cell wall-associated proteins contribute to regions of flexibility and rigidity within the cell wall, allowing for remodeling when necessary during growth, environmental adaptation, or stress response activation. These polysaccharide interactions are vital to plant growth, however they also contribute to the level of difficulty encountered when attempting to analyze cell wall structure and composition. In the past, ...
[摘要] [摘要] 植物细胞壁是由多糖和蛋白质组成的复杂网络，可为植物细胞提供强度和结构完整性，并且在生长，发育和防御反应中起着至关重要的作用。细胞壁多糖可大致分为三类：纤维素，果胶和半纤维素。多糖和细胞壁相关蛋白之间的动态相互作用有助于细胞壁内的柔韧性和刚性区域，从而在生长，环境适应或应激反应激活过程中在必要时进行重塑。这些多糖相互作用对于植物生长至关重要，但是它们也增加了植物的难度。 在尝试分析细胞壁结构和组成时进行了反驳。过去，已经使用冗长的方案来量化对纤维素有贡献的细胞壁单糖以及中性和酸性细胞壁多糖。近来，STREA 毫升为单糖定量描述INED方法。该方案结合了简化的水解方法，随后进行了多次运行的高性能阴离子交换色谱和脉冲安培检测（HPAEC-PAD）。在这里，我们介绍了该协议的更新版本，其中我们可以在单个高效液相色谱HPAEC-PAD梯度曲线中分析所有九种细胞壁单糖。包括酶促淀粉降解以及替代的内部标准以提高定量准确性，以及便于数据分析的即用型Python脚本为该协议增加了广泛的应用范围。该方案用于分析拟南芥的浅色幼苗和深色的胚轴，但适用于任何植物组织。
[背景] ...
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