|
Author:
Date:
2017-03-05
[Abstract] Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For these reasons, studying the properties of lignin is of great interest to researchers in agriculture and bioenergy fields. This protocol describes the acetyl bromide method of total lignin extraction and quantification, which is favored among other methods for its high recovery, consistency, and insensitivity to different tissue types (Johnson et al., 1961; Chang et al., 2008; Moreira-Vilar et al., 2014; Kapp et al., ...
[摘要] 木质素是地球上第二大丰富的生物聚合物，为植物在二次细胞壁提供机械支持，防止非生物和生物胁迫。然而，木质素还可以作为生物燃料生成的糖化的障碍（Carroll和Somerville，2009; Zhao和Dixon，2011; Wang等人，2013）。由于这些原因，研究木质素的性质对于农业和生物能源领域的研究人员非常感兴趣。该方案描述了总木素提取和定量的乙酰溴方法，其对于其不同组织类型的高回收率，一致性和不敏感性的其它方法是有利的（Johnson等人，1961; Chang < em等人，2008;="" moreira-vilar等人，2014;="">
背景 用于从植物生物量定量木质素的乙酰溴方法已被用于精确测量几十年的总木质素含量（Johnson等，1961）。最近，与替代的巯基乙酸和Klason木质素方法（Moreira-Vilar等人，2014））相比，该方法获得了木质素定量的最佳方法的支持。这三种方法的比较经验证明，乙酰溴方法一直导致木质素回收率最高，对组织类型，木质化程度和木质素组成不敏感（Moreira-Vilar等人）。 ...
|
|
Author:
Date:
2016-10-20
[Abstract] The plant cell wall is primarily composed of the polysaccharides cellulose, hemicellulose and pectin. The structural and compositional complexity of these components are important for determining cell wall function during plant growth. Moreover, cell wall structure defines a number of functional properties of plant-derived biomass, such as rheological properties of foods and feedstock suitability for the production of cellulosic biofuels. A typical characterization of cell wall chemistry in the molecular biology lab consists of a mild acid hydrolysis for the quantification of hemicellulose and pectin-derived monomers and a separate analysis of cellulose by the Updegraff method. We have adopted a streamlined ‘one-step two-step’ hydrolysis protocol that allows for the simultaneous ...
[摘要] 植物细胞壁主要由多糖纤维素，半纤维素和果胶组成。这些组分的结构和组成复杂性对于确定植物生长期间的细胞壁功能是重要的。此外，细胞壁结构限定了植物来源的生物质的多种功能性质，例如食品的流变性质和用于生产纤维素生物燃料的原料适用性。分子生物学实验室中细胞壁化学的典型表征包括用于定量半纤维素和果胶衍生单体的温和酸水解和通过Updegraff方法对纤维素的单独分析。我们采用了一个简化的"一步两步"水解方案，允许通过配对的脉冲安培检测（HPAEC-PAD）的高效阴离子交换层析同时测定纤维素含量，中性糖和糖醛酸样品。在我们的工作中，该方案已经在很大程度上替代了Updegraff纤维素定量和用2μMTFA水解以在微量级上测定基质多糖组成。
[背景] 是基于在121℃下在4％（w/v）硫酸中水解的样品的配对分析。一组样品首先用72％（w/w）硫酸预处理以使纤维素膨胀并使其易于稀释酸水解（图1中的Saeman水解; ...
|