{{ 'Home' | translate }} {{'About us' | translate}} {{'Sponsors' | translate}}
 
{{'Search' | translate}}
 

Calcofluor White Stain

Calcofluor白色污渍
Company: Sigma-Aldrich
Catalog#: 18909
Bio-protocol()
Company-protocol()
Other protocol()
Aniline Blue and Calcofluor White Staining of Callose and Cellulose in the Streptophyte Green Algae Zygnema and Klebsormidium
Author:
Date:
2016-10-20
[Abstract]  Plant including green algal cells are surrounded by a cell wall, which is a diverse composite of complex polysaccharides and crucial for their function and survival. Here we describe two simple protocols to visualize callose (1→3-β-D-glucose) and cellulose (1→4-β-D-glucose) and related polysaccharides in the cell walls of streptophyte green algae. Untreated or algal cells heated in NaOH are incubated in Calcofluor white (binding to β-glucans including cellulose) or Aniline blue (binding to callose), respectively. Both dyes can be visualized by epifluorescence microscopy. [摘要]  包括绿藻细胞的植物被细胞壁包围，所述细胞壁是复合多糖的多样复合物，并且对于它们的功能和存活是至关重要的。在这里我们描述两个简单的协议，可视化胼lose质（1→3-β-D-葡萄糖）和纤维素（1→4-β-D-葡萄糖）和相关的多糖在链霉素绿藻的细胞壁。将在NaOH中加热的未处理或藻类细胞分别在Calcofluor白（结合β-葡聚糖包括纤维素）或苯胺蓝（结合胼cal质）中孵育。两种染料都可以通过落射荧光显微镜观察。

[背景] 由于其容易和快速的适用性，使用苯胺蓝显现各种株Klebsormidium sp中的胼。质。和在更艰苦的固定和免疫定位方案（Herburger和Holzinger，2015）之前使用Zygnema。应用苯胺蓝染色和单克隆抗体对胼lose质产生类似的结果（Herburger和Holzinger，2015）。 Calcofluor白色染色是观察包括细胞壁的纤维素的1→4-β-葡聚糖部分的最快方式，因为不需要预处理。

Comments



twitter facebook linkedin
{{'About us' | translate}}
 
{{'Contact US' | translate}}

©  Bio-protocol LLC.
Terms of Service | Copyright IP Policy