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Author:
Date:
2016-10-05
[Abstract] Blood serum or plasma osmolality is the measure of electrolyte to water balance in the body’s circulation, and is tightly regulated in physiological states in order to maintain normal levels of serum solute (Bourque, 2008). Osmolality is defined as the number of osmoles of solute per kg of water (mOsm/kg) (Dufour, 1993) and can be measured using different techniques that rely on the colligative properties of the solution. The most commonly used in lab settings are vapour pressure and freezing point osmometry, which are relatively quick and easy to perform. Freezing point osmometry is preferred because it is insensitive to volatile compounds, such as alcohol, that may be present in the solution. Measurement of serum or plasma osmolality is clinically relevant for a number of conditions and ...
[摘要] 血清或血浆渗透压是体内循环中电解质与水平衡的量度，并且在生理状态下受到严格的调节，以维持血清溶质的正常水平（Bourque，2008）。重量摩尔渗透压浓度定义为每公斤水溶质渗透摩尔数（mOsm / kg）（Dufour，1993），并且可以使用依赖于溶液的碰撞性质的不同技术来测量。实验室设置中最常用的是蒸气压和凝固点渗透压测量法，它们相对快速且易于执行。凝固点渗透压是优选的，因为它对溶解中可能存在的挥发性化合物（例如醇）不敏感。血清或血浆渗透压的测量在许多病症和疾病方面具有临床意义，包括高钠血症，糖尿病酮症酸中毒和不良抗生素综合征（Ellison，2013; Lupsa和Inzucchi，2013; Reddi，2013）。在本协议中，我们描述了使用冰点渗透压测定技术测量大鼠血清渗透压的方法，原先在我们以前在败血症中的渗透调节扰动研究中概述（Stare等，2015）。
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