Kapa HiFi polymerase
Company: Roche Diagnostics
Catalog#: KK2101
Product evaluation:
Determination of Mutation Frequency During Viral DNA Replication
[Abstract]  This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. In the original Carrascosa et al. (1982), the protocol was carried out with two cloned viruses, BA71Vc (a purified clone from BA71V wild type strain) and vΔpolX (lacking the reparative polymerase, pol X, gene), and two different cell types ...

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