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Author:
Date:
2016-10-05
[Abstract] In response to pathogen infection and tissue damage, inflammasome sensors such as NLRP3 and AIM2 are activated, which triggers PYRIN domain (PYD)-mediated ASC nucleation, followed by self-perpetuating ASC polymerization, which ultimately culminates in caspase-1 activation, interleukin (IL)-1β and IL-18 processing and release and pyroptosis (Ratsimandresy et al., 2013; Cai et al., 2014). Inflammasomes release not only cytokines, but also the polymeric ASC danger particles (pASC) by pyroptosis, which perpetuate and propagate inflammasome responses to bystander cells to engage cell intrinsic ASC and caspase-1 (Baroja-Mazo et al., 2014; Franklin et al., 2014). In this protocol we describe intraperitoneal injection of polymeric ASC particles as a danger ...
[摘要] 响应病原体感染和组织损伤，激活炎症小体传感器如NLRP3和AIM2，其触发PYRIN结构域（PYD）介导的ASC成核，随后是自我永存的ASC聚合，其最终终止于胱天蛋白酶-1激活，白细胞介素 IL）-1β和IL-18加工和释放和pyroptosis（Ratsimandresy等人，2013; Cai等人，2014）。 Inflammasomes不仅释放细胞因子，而且还通过pyroptosis释放聚合的ASC危险颗粒（pASC），其使得对旁观者细胞的炎症反应永久化并增殖，从而使细胞内在ASC和胱天蛋白酶-1参与（Baroja-Mazo等人 。，2014; Franklin 。。，2014）。 在该方案中，我们描述了腹膜内注射聚合物ASC颗粒作为危险信号，并通过ELISA在腹膜灌洗中测量嗜中性粒细胞浸润和促炎细胞因子IL-1β的水平（de Almeida等人， 2015）。
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