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Tetramethylrhodamine-5-(and-6)-Isothiocyanate (5(6)-TRITC), mixed isomers

四甲基罗丹明-5-(和-6) - 硫代异氰酸酯(5(6)-TRITC),混合异构体

Company: Thermo Fisher Scientific
Catalog#: T490
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Skin TRITC Painting to Track Dendritic Cells Migrating to the Lymph Nodes
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2016-09-20
[Abstract]  Our protocol describes a simple method that allows tracking of dendritic cells (DC) migration from the flank skin to draining lymph nodes (LN) using a red fluorescent dye tetramethylrhodamine-5-isothiocyanate (TRITC). TRITC is a photostable dye that readily labels cells including DC in the skin and can survive repeated exposure of two-photon laser excitation for prolonged imaging duration. This method can be combined with various fluorescent antibody labels or transgenic mouse strains (such as CD11c-EYFP) to visualize distinct DC populations simultaneously. [摘要]  我们的协议描述了一个简单的方法,允许跟踪树突状细胞(DC)从侧面皮肤迁移到引流淋巴结(LN)使用红色荧光染料四甲基罗丹明-5-异硫氰酸酯(TRITC)。 TRITC是光稳定染料,其容易地标记包括皮肤中的DC的细胞,并且可以在双光子激光激发的重复暴露下存活长时间的成像持续时间。 该方法可以与各种荧光抗体标记或转基因小鼠品系(例如CD11c-EYFP)组合以同时可视化不同的DC群体。

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