Author:
Van T. Luu, Melissa Stiebner, Paula Emmerich Maldonado, Sandra Valdés, Didier Marín, Gerardo Delgado, Virginia Laluz, Lin-Bo Wu, Paul Chavarriaga, Joe Tohme, Inez H. Slamet-Loedin and Wolf B. Frommer,
Date:
2020-09-05
[Abstract] Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of the world’s population. As a result, numerous transformation methods have been developed for both indica and japonica rice. Since breeders continuously develop new rice varieties, transformation protocols have to be adapted for each new variety. Here we provide an optimized transformation protocol with detailed tips and tricks for a new African variety Komboka using immature embryos. In Komboka, we obtained an apparent transformation rate of up to 48% for GUS/GFP reporter gene constructs using this optimized protocol. This ...
[摘要] [摘要 ] 遗传转化对于研究基因功能和农作物工程引入新性状均至关重要。水稻(Oryza sativa L.)是植物研究中的重要模型,因为它是世界一半以上人口的主食。其结果是,大量的转化方法已经开发了两个籼稻和粳稻米。由于育种者不断开发新的水稻品种,因此必须针对每个新品种适应转化方案。在这里,我们为使用未成熟胚的非洲新品种Komboka 提供了一种优化的转化协议,包括详细的技巧和窍门。在Komboka中,我们使用此优化方案对GUS / GFP报告基因构建体的表观转化率高达48%。该协议也适用于其他优质lite 稻品种。
[背景 ] 为植物的遗传转化的各种方法公顷一直在开发,例如穿孔(Shimamoto PEG介导的原生质体转染。等人,1989;达塔。等人,1992),生物射弹转化(Christou的等人,1991)和农杆菌-介导的转化(Slamet-Loedin 等,2014)。农杆菌介导的转化是将DNA引入植物的最广泛使用的方法之一(van Wordragen and Dons,1992)。该方法已被广泛用于研究,并已成为生物技术的关键先决条件。自从开发新的育种技术(如基因组编辑)以来,它就变得越来越重要(Char ...
|