| Isolation and Culture of Neurospheres for the Study of Pathogenesis of Prion Disease
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Author:
Date:
2014-03-20
[Abstract] Neurosphere contains neural stem cells that are capable of self-renewal and multilineage differentiation including neurons, astrocytes, and oligodendrocytes (Gage, 2000). Cell culture model using differentiated neurosphere cultures are suggested to be a valuable tool for studying the pathogenesis of prion disease at the cellular level (Iwamaru et al., 2013). This protocol describes the procedure for a culture of whole brain-derived neurospheres from newborn mouse brains. Neurosphere formation steadily occurs within a week from the cultures of neonatal whole brains and these cells have stem cell properties.
[摘要] 神经球包含能够自我更新和多向分化的神经干细胞，包括神经元，星形胶质细胞和少突胶质细胞（Gage，2000）。 使用分化的神经球培养物的细胞培养模型被认为是用于研究朊病毒疾病在细胞水平的发病机制的有价值的工具（Iwamaru等人，2013）。 该协议描述了来自新生小鼠脑的全脑衍生的神经球的培养的程序。 神经球形成稳定地发生在新生儿全脑的培养物的一周内，并且这些细胞具有干细胞特性。
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| Isolation of Cancer Epithelial Cells from Mouse Mammary Tumors
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Author:
Date:
2013-02-05
[Abstract] The isolation of cancer epithelial cells from mouse mammary tumor is accomplished by digestion of the solid tumor. Red blood cells and other contaminates are removed using several washing techniques such that primary epithelial cells can further enriched. This procedure yields primary tumor cells that can be used for in vitro tissue culture, fluorescence-activated cell sorting (FACS) and a wide variety of other experiments (Lo et al., 2012).
[摘要] 通过消化实体瘤完成从小鼠乳腺肿瘤中分离癌上皮细胞。 使用几种洗涤技术除去红细胞和其它污染物，使得原代上皮细胞可以进一步富集。 该程序产生可用于体外组织培养，荧光激活细胞分选（FACS）和各种其它实验的原发性肿瘤细胞（Lo等人， ，2012）。
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