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Whatman® quantitative filter paper, ashless, Grade 40

Whatman ®定量滤纸，无灰，40级
Company: Sigma-Aldrich
Catalog#: Z241261
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A Method to Analyze Local and Systemic Effects of Environmental Stimuli on Root Development in Plants
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2016-09-05
[Abstract]  Root development in vascular plants is innately tied to the environment. However, relatively little attention has been paid toward understanding the spatial scales at which the root perceives and responds to external stimuli. While some environmental signals elicit global responses that affect root system architecture, others may have more localized effects. We have observed that various developmental processes can be induced or suppressed along the circumference of the main root depending on local contact with available water in a process termed hydropatterning (Bao et al., 2014). Our studies of hydropatterning indicate that the root can detect and respond to certain external stimuli at the resolution of the diameter of a single organ. In order to characterize developmental ... [摘要]  维管植物中的根发育与环境固有关系。然而，相对较少的关注已经被用于理解根部感知和响应外部刺激的空间尺度。虽然一些环境信号引起影响根系统结构的全局响应，但其他环境信号可能具有更局部化的效应。我们已经观察到，根据在称为水图案化的过程中与可用水的局部接触，可以沿着主根的圆周诱导或抑制各种发育过程（Bao等人，2014）。我们对水印的研究表明根部可以检测和响应某些外部刺激在单个器官的直径的分辨率。为了表征在该空间尺度的发育图案化，我们开发了使用琼脂培养基在体外改变穿过根的周向轴的环境输入的程序。根在"三明治"中的两块琼脂培养基之间生长。局部环境条件可以根据任一侧的介质的组成而变化。局部作用的刺激可以与基于根的发育反应系统作用的刺激相区分。在这里我们描述了整体的方法，并提供了一个例子，它如何可以用于分析外部水势梯度响应中玉米Zea mays（玉米）的侧根图案。我们还讨论了该方法如何可以更广泛地用于其他植物物种和环境处理。

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