| Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
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Author:
Date:
2018-08-05
[Abstract] This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding gene, the method enables to clearly distinguish the cells retaining fluorescent signals from other anther cells. It is applicable to both live and fixed samples, and presumably to other plant tissues.
[摘要] 该方案演示了一种确定荧光标记蛋白在水稻发育花药的振动切片上的亚细胞定位的简单方法。 如果使用细胞类型特异性启动子来驱动标记的蛋白质编码基因，该方法能够清楚地区分保留荧光信号的细胞与其他花药细胞。 它适用于活的和固定的样品，并且可能适用于其他植物组织。
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| Olfactory Bulb (OB) Transplants
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Author:
Date:
2016-09-05
[Abstract] Transplantation in mouse brain slices is a powerful tool in order to study axon targeting and migrational events during development. Taking advantage of donors and recipients belonging to different genotypes, this technique allows researchers to assess the contribution of donor and/or recipient tissue by performing various combinations and to study cell-autonomous functions or effects that are influenced by the recipient’s environment (Bastakis, et al., 2015). Here we describe the transplantation procedure on sagittal brain slices containing olfactory bulb (OB). Specifically, we have transplanted the proximal-to-the-cortex part of dorsal OB to the same region on a recipient slice. Transplanted slices can be cultured for up to 3 days before their morphology is disfigured due to ...
[摘要] 小鼠脑切片中的移植是一个强大的工具，以便研究轴突靶向和发展过程中的迁移事件。 利用属于不同基因型的供体和受体，该技术允许研究人员通过进行各种组合来评估供体和/或受体组织的贡献，并研究受受体环境影响的细胞自主功能或效应（Bastakis， em>等人，2015）。 在这里我们描述的矢状大脑切片包含嗅球（OB）的移植程序。 具体来说，我们已经将背侧OB的近端到外皮部分移植到受体切片上的相同区域。 移植的切片可以培养长达3天，之后由于3D中的生长它们的形态被破坏。 这些切片的重新切片允许更详细的免疫组织化学分析。
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