MS-SAFE Protease and Phosphatase Inhibitor

MS-SAFE蛋白酶和磷酸酶抑制剂

Company: Sigma-Aldrich

Catalog#: MSSAFE

Single Oligonucleotide Capture of RNA And Temperature Elution Series (SOCRATES) for Identification of RNA-binding Proteins

Allen T. Yu, Disha Aggarwal, Darryl Pappin and David L. Spector

Published online: 2022-12-20

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Identification of RNA-binding Proteins

Author:

Kazuya Masuda and Tadamitsu Kishimoto,

Date:

2016-09-05

[Abstract] This protocol describes the extraction of RNA-binding proteins (RBPs) from cell lysates. In order to pull down target RBPs, 5-bromo-UTP (BrUTP)-incorporated RNA probes are used, which are generated by in vitro transcription. The schematic diagram (Flowchart) with procedure is indicated (Figure1 and Figure 2).

Figure 1. Schematic diagram of procedure (A-H). Flow chart of experimental procedure is indicated at A-H.

Figure 2. Linearization of plasmids by restricted enzyme. The plasmid is cut at restriction sites adjacent to its ... [摘要] 该协议描述了从细胞裂解物中提取RNA结合蛋白（RBP）。为了下拉靶标RBP，使用通过体外转录产生的含有5-溴-UTP（BrUTP）的RNA探针。示意图（流程图）与过程（图1和图2）。

图1.程序示意图（AH）。实验程序的流程图在AH指示。

图2.质粒的线性化限制性酶。在与其克隆元件相邻的限制性位点切割质粒。

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MS-SAFE Protease and Phosphatase Inhibitor

MS-SAFE蛋白酶和磷酸酶抑制剂

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