| Transplantation of Embryonic Cortical Tissue into Lesioned Adult Brain in Mice
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Author:
Date:
2017-06-20
[Abstract] Transplantation of embryonic cortical tissue for repairing the damaged brain has provided a potential therapy for brain injury and diseases. The grafted tissue can successfully survive and participate in reestablishing the functional neural circuit of the host brain. Transplantation surgery can be combined with fluorescently labeled transgenic mice to evaluate the reconstruction of neuronal network (Falkner et al., 2016) and the repopulation of a subset of cortical cells. By using this approach, we have shown that infiltrating cells from host brain can restore the microglial population in the graft tissue (Wang et al., 2016). This protocol describes the detailed procedure of the transplantation surgery in mice, including establishing a lesion model in the host brain, ...
[摘要] 移植胚胎皮质组织修复损伤的大脑已经为脑损伤和疾病提供了潜在的治疗方法。 移植组织可以成功地存活并参与重建宿主大脑的功能性神经回路。 移植手术可以与荧光标记的转基因小鼠结合，以评估神经元网络的重建（Falkner等，2016）和皮质细胞亚群的再次增殖。 通过使用这种方法，我们已经显示来自宿主大脑的浸润细胞可以恢复移植组织中的小胶质细胞群体（Wang等，2016）。 该方案描述了小鼠移植手术的详细步骤，包括在宿主脑中建立病变模型，制备胚胎皮质移植物，并将胚胎皮质移植物移植到成年大脑中。
【背景】成年大脑中的大多数神经元是后有丝分裂细胞，并且不能再生新的子细胞，这导致在患有脑损伤或疾病后自我修复成人脑的能力有限。用胚胎神经移植代替损伤的脑组织是修复成人大脑受损神经通路的潜在有效疗法之一（Tuszynski，2007）。自20世纪70年代以来，这个研究领域引起了很多关注（Das和Altman，1972; Bjorklund和Stenevi，1979年），在过去三十年中取得了显着的成功。这些研究表明，移植组织中的神经元可以成功地在宿主大脑中存活，并开发传播预测以重建宿主和供体神经元之间的突触连接（Gaillard和Roger，2000; Gaillard等，2004; Gaillard，2007; Gaillard et al ，2007; Falkner et ...
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| Salinity and Drought Treatment Assays in Kenaf (Hibiscus cannabinus L.)
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Author:
Date:
2016-09-05
[Abstract] Salinity and drought are the two main factors that cause fiber yield and quality losses in kenaf. It is reported that salinity and drought can affect more than 10% of arable land and cause a global decline in the average yields of major crops by more than 50%. Therefore, understanding plant tolerance of drought and salinity is of fundamental importance and has become the focus of intensive research. This protocol describes a simple and reproducible protocol to imitate natural salinity and drought stress under soil conditions. Even though the water-culture method is most frequently used for salinity and drought treatments, the soil-culture method in this study was more applicable to studying natural stress conditions.
[摘要] 盐度和干旱是导致红麻产量和质量损失的两个主要因素。 据报道，盐度和干旱可影响10％以上的耕地，导致主要作物平均单产下降50％以上。 因此，了解植物对干旱和盐分的耐受性具有根本重要性，已成为深入研究的重点。 该协议描述了在土壤条件下模拟天然盐度和干旱胁迫的简单和可重复的方案。 即使水培法最常用于盐分和干旱处理，本研究中的土壤培养方法更适用于研究自然胁迫条件。
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