{{'Search' | translate}}
 

PrecisionGlideTM Needle 16G x 1 1/2"

常用注射针头

Company: BD
Catalog#: 305198
Bio-protocol()
Company-protocol()
Other protocol()

Assessing Classical Olfactory Fear Conditioning by Behavioral Freezing in Mice
Author:
Date:
2018-09-20
[Abstract]  Classical fear conditioning typically involves pairing a discrete cue with a foot shock. Quantifying behavioral freezing to the learned cue is a crucial assay for neuroscience studies focused on learning and memory. Many paradigms utilize discrete stimuli such as tones; however, given mice are odor-driven animals and the wide variety of odorants commercially available, using odors as conditioned stimuli presents advantages for studies involving learning. Here, we describe detailed procedures for assembling systems for presenting discrete odor cues during single-day fear conditioning and subsequent analysis of freezing behavior to assess learning. [摘要]  经典的恐惧条件反射通常涉及将离散的线索与脚部震动配对。 量化对学习线索的行为冻结是针对学习和记忆的神经科学研究的关键分析。 许多范例利用诸如音调之类的离散刺激; 然而,鉴于小鼠是气味驱动的动物和商业上可获得的各种气味剂,使用气味作为条件刺激对于涉及学习的研究具有优势。 在这里,我们描述了用于组装系统的详细程序,该系统用于在单日恐惧条件反射期间呈现离散的气味线索并随后分析冷冻行为以评估学习。

【背景】联想恐惧学习是几种焦虑症的根源,涉及将中性刺激与厌恶结果配对。这种配对产生强烈的行为恐惧反应,以冷冻(LeDoux,2003)的形式,对条件刺激,可以量化为恐惧学习和记忆的量度。离散刺激,如音调,通常被用作恐惧条件反射的条件刺激;然而,嗅觉提示在诱导学习冷冻方面也非常有效(Pavesi et al。,2012; Ross and Fletcher,2018)。这种联想恐惧学习方法不同于利用捕食者气味的方法,这种方法产生本能行为而非学习行为,使其成为快速评估嗅觉学习的理想选择。行为冻结,定义为缺乏所有自愿运动(Blanchard和Blanchard,1969; ...

A Microfluidic Device for Massively Parallel, Whole-lifespan Imaging of Single Fission Yeast Cells
Author:
Date:
2018-04-05
[Abstract]  Whole-lifespan single-cell analysis has greatly increased our understanding of fundamental cellular processes such as cellular aging. To observe individual cells across their entire lifespan, all progeny must be removed from the growth medium, typically via manual microdissection. However, manual microdissection is laborious, low-throughput, and incompatible with fluorescence microscopy. Here, we describe assembly and operation of the multiplexed-Fission Yeast Lifespan Microdissector (multFYLM), a high-throughput microfluidic device for rapidly acquiring single-cell whole-lifespan imaging. multFYLM captures approximately one thousand rod-shaped fission yeast cells from up to six different genetic backgrounds or treatment regimens. The immobilized cells are fluorescently imaged for over a ... [摘要]  整个寿命的单细胞分析极大地增加了我们对细胞老化等基本细胞过程的理解。为了观察整个寿命期间的个体细胞,必须从生长培养基中移除所有后代,通常通过手动显微切割。然而,手动显微切割费力,低通量,并且与荧光显微镜不兼容。在这里,我们描述了多路复用裂变酵母寿命显微解剖器(multFYLM)的组装和操作,这是一种用于快速获取单细胞全寿命成像的高通量微流体装置。 multFYLM从多达六种不同的遗传背景或治疗方案中捕获约一千个杆状裂殖酵母细胞。将固定的细胞荧光成像超过一周,而将子代细胞从装置中取出。得到的数据集产生记录每个细胞复制寿命的高分辨率多通道图像。我们预计multFYLM将广泛适用于裂殖酵母(Schizosaccharomyces pombe)和其他对称分裂的单细胞生物的单细胞整个寿命研究。

【背景】细胞衰老导致细胞功能的累积下降,最终导致死亡。大多数关于细胞衰老的研究侧重于模型单细胞生物的复制寿命,例如出芽酵母酿酒酵母(Nyström和Liu,2014; Wasko和Kaeberlein,2014; Wierman和Smith,2014; Ruetenik和Barrientos ,2015)。细胞的复制寿命(RLS)被定义为母细胞在其生命过程中产生的女儿的数量(Henderson和Gottschling,2008; Sutphin等人,2014)。 ...

LT-HSC Methylcellulose Assay
Author:
Date:
2014-03-05
[Abstract]  Hematopoietic differentiation is a highly complex process originating from an extraordinary population of cells called long-term repopulating hematopoietic stem cells (LT-HSCs). The unique feature of all stem cells, including HSCs, is their exceptional ability to divide asymmetrically giving rise to two different kinds of offspring. One daughter cell becomes an LT-HSC itself (self-renews) to maintain the LT-HSC pool, whereas the second daughter cell pursues a differentiation fate to ultimately give rise to terminally differentiated mature blood cells (Orkin and Zon, 2008). Quantification of phenotypic LT-HSCs can be performed by multi-color flow cytometry and the gold standard for assessment of LT-HSC self-renewal and function is competitive bone marrow transplantation (Miller et al. ... [摘要]  造血分化是一种非常复杂的过程,源自称为长期重建造血干细胞(LT-HSCs)的非凡细胞群。所有干细胞(包括HSC)的独特之处在于其不对称的划分能够产生两种不同种类的后代。一个子细胞成为LT-HSC本身(自我更新)以维持LT-HSC库,而第二个子细胞追求分化命运,最终产生终末分化的成熟血细胞(Orkin和Zon,2008)。表型LT-HSC的定量可以通过多色流式细胞术进行,用于评估LT-HSC自我更新和功能的黄金标准是竞争性骨髓移植(Miller等,2008)。尽管这些方法对于确定LT-HSC丰度和功能是不可替代的,但它们具有其缺点和局限性。例如,竞争性骨髓移植通常被监测为12-16周内外周血献血者贡献的函数。虽然减少的外周血供体贡献本身表示干/祖细胞隔室中的损伤,但是它不能明确区分降低的LT-HSC自我更新,受损的LT-HSC分化或受损的祖细胞分化。这里我们描述一种LT-HSCs甲基纤维素集落形成测定法,作为直接评估LT-HSC分化能力的快速互补体外方法。如Kerenyi等人所述(2013),该技术是区分LT-HSC或祖细胞分化缺陷的有力工具。

Comments