Dulbecco’s Modified Eagle’s Medium - low glucose
Company: Sigma-Aldrich
Catalog#: D5523
Product evaluation:
Assessing Mitochondrial Transport via Cytoplasmic Nanotubular Bridges in Cells
Author:
Date:
2015-08-05
[Abstract]  This protocol aims to study intercellular transport of mitochondria, dynamic cellular organelles via tunnelling nanotubes (TNT), a cell membrane extension of cytoskeletal elements. The nanotubular bridges or the tunnelling nanotube highways are one of the emerging new cell-to-cell communication systems which mediates exchange of cellular materials, most importantly as in our observation, mitochondria. Mesenchymal stem cells (MSC) have been well studied to be endowed with a highly efficient ...

Determination of Mutation Frequency During Viral DNA Replication
Author:
Date:
2014-03-20
[Abstract]  This protocol is a simple method for evaluating mutation frequency during African swine fever virus (ASFV) replication, although it could be used also for other DNA viruses (poxvirus, herpesvirus, mimivirus, etc) with minor modifications. In the original Carrascosa et al. (1982), the protocol was carried out with two cloned viruses, BA71Vc (a purified clone from BA71V wild type strain) and vΔpolX (lacking the reparative polymerase, pol X, gene), and two different cell types ...

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