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Author:
Date:
2015-06-20
[Abstract] Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis pollen tubes followed by a sugar composition analysis by gas chromatography mass spectrometry.
[摘要] 用热水提取是用于从醇不溶性残余物提取物中回收果胶的最古老和最简单的方法，尽管该方法尚未广泛用于花粉管的细胞壁分析，所述花粉管是用于研究细胞壁的模型。 该方案描述了该方法应用于从6h-旧拟南芥花粉管中提取果胶，然后通过气相色谱质谱法进行糖组成分析。
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